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Commit 3baf5449 authored by Yuhao Lin's avatar Yuhao Lin
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修改section2内容为Double Enzyme DNA Digestion& Agarose Gel Electrophoresis

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......@@ -230,12 +230,26 @@ LB selective medium (Chloramphenicol)<br />
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<h2>LB medium preparation</h2>
<h2>Double Enzyme DNA Digestion& Agarose Gel Electrophoresis</h2>
<p>Content for section 2.</p>
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<h4 className="center-text">LB medium preparation</h4>
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<p className="indent">las ijffs aiskfd fskj iiwls asd.aass ffas awssd awus iisal fask.aisisad ksjdfkaf iwjasifjakdshf wijdfalksjf wiksjkfjksalhf, gsahfjhgejkfh uhaejkfh sjdihgfuqiw jh sjiafhjsaj fh asd.</p>
<h4 className="center-text">Double Enzyme DNA Digestion& Agarose Gel Electrophoresis</h4>
<p className="indent">(1)Material<br />
Plasmid DNA<br />
10× FastDigest or 10× FastDigest Green Buffer<br />
Double Distillation Water (ddH2O)<br />
FastDigest enzyme<br />
Alkaline phosphatase<br />
</p>
<p className="indent">1. Add 1μg plasmid DNA, 1μl FastDigest enzyme, 2μl 10× FastDigest or 10× FastDigest<br />
2. Green Buffer and ddH2O to 20μl into an PCR tube.<br />
3. Put the tube into the oven at 37℃ for 1h to 1.5h.<br />
4. Add 1μl Alkaline phosphatase.<br />
5. Put the tube into the oven at 37℃ for 0.5h.<br />
6. Stop the reaction by placing the tube at -4°C for 5 to 10 minutes.<br />
7. Agarose Gel Electrophoresis.<br />
8. Store the tube into the refrigerator at -20℃.<br />
</p>
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