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2024 Competition
SMU-GDMU-CHINA
Commits
3baf5449
Commit
3baf5449
authored
5 months ago
by
Yuhao Lin
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修改section2内容为Double Enzyme DNA Digestion& Agarose Gel Electrophoresis
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#508898
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5 months ago
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Stage: deploy
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src/contents/experiments.tsx
+18
-4
18 additions, 4 deletions
src/contents/experiments.tsx
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src/contents/experiments.tsx
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3baf5449
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@@ -230,12 +230,26 @@ LB selective medium (Chloramphenicol)<br />
onMouseEnter
=
{
()
=>
handleMouseEnter
(
'
section2
'
)
}
style
=
{
{
display
:
visibleSection
===
'
section2
'
?
'
block
'
:
'
none
'
}
}
>
<
h2
>
LB medium preparation
</
h2
>
<
h2
>
Double Enzyme DNA Digestion
&
Agarose Gel Electrophoresis
</
h2
>
<
p
>
Content for section 2.
</
p
>
<
div
className
=
"rounded-border"
>
<
h4
className
=
"center-text"
>
LB medium preparation
</
h4
>
<
p
className
=
"indent"
>
las ijffs aiskfd fskj iiwls asd.aass ffas awssd awus iisal fask.aisisad ksjdfkaf iwjasifjakdshf wijdfalksjf wiksjkfjksalhf, gsahfjhgejkfh uhaejkfh sjdihgfuqiw jh sjiafhjsaj fh asd.
</
p
>
<
p
className
=
"indent"
>
las ijffs aiskfd fskj iiwls asd.aass ffas awssd awus iisal fask.aisisad ksjdfkaf iwjasifjakdshf wijdfalksjf wiksjkfjksalhf, gsahfjhgejkfh uhaejkfh sjdihgfuqiw jh sjiafhjsaj fh asd.
</
p
>
<
h4
className
=
"center-text"
>
Double Enzyme DNA Digestion
&
Agarose Gel Electrophoresis
</
h4
>
<
p
className
=
"indent"
>
(1)Material
<
br
/>
Plasmid DNA
<
br
/>
10× FastDigest or 10× FastDigest Green Buffer
<
br
/>
Double Distillation Water (ddH2O)
<
br
/>
FastDigest enzyme
<
br
/>
Alkaline phosphatase
<
br
/>
</
p
>
<
p
className
=
"indent"
>
1. Add 1μg plasmid DNA, 1μl FastDigest enzyme, 2μl 10× FastDigest or 10× FastDigest
<
br
/>
2. Green Buffer and ddH2O to 20μl into an PCR tube.
<
br
/>
3. Put the tube into the oven at 37℃ for 1h to 1.5h.
<
br
/>
4. Add 1μl Alkaline phosphatase.
<
br
/>
5. Put the tube into the oven at 37℃ for 0.5h.
<
br
/>
6. Stop the reaction by placing the tube at -4°C for 5 to 10 minutes.
<
br
/>
7. Agarose Gel Electrophoresis.
<
br
/>
8. Store the tube into the refrigerator at -20℃.
<
br
/>
</
p
>
</
div
>
</
Element
>
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