diff --git a/src/contents/experiments.tsx b/src/contents/experiments.tsx index 53c4ca13f913f61b59e2f8aa1aa7bd63d1a37141..7fe5ad200d041c407ed9bfab3b454d2dd6d021d4 100644 --- a/src/contents/experiments.tsx +++ b/src/contents/experiments.tsx @@ -230,12 +230,26 @@ LB selective medium (Chloramphenicol)<br /> onMouseEnter={() => handleMouseEnter('section2')} style={{ display: visibleSection === 'section2' ? 'block' : 'none' }} > - <h2>LB medium preparation</h2> + <h2>Double Enzyme DNA Digestion& Agarose Gel Electrophoresis</h2> <p>Content for section 2.</p> <div className="rounded-border"> - <h4 className="center-text">LB medium preparation</h4> - <p className="indent">las ijffs aiskfd fskj iiwls asd.aass ffas awssd awus iisal fask.aisisad ksjdfkaf iwjasifjakdshf wijdfalksjf wiksjkfjksalhf, gsahfjhgejkfh uhaejkfh sjdihgfuqiw jh sjiafhjsaj fh asd.</p> - <p className="indent">las ijffs aiskfd fskj iiwls asd.aass ffas awssd awus iisal fask.aisisad ksjdfkaf iwjasifjakdshf wijdfalksjf wiksjkfjksalhf, gsahfjhgejkfh uhaejkfh sjdihgfuqiw jh sjiafhjsaj fh asd.</p> + <h4 className="center-text">Double Enzyme DNA Digestion& Agarose Gel Electrophoresis</h4> + <p className="indent">(1)Material<br /> +Plasmid DNA<br /> +10× FastDigest or 10× FastDigest Green Buffer<br /> +Double Distillation Water (ddH2O)<br /> +FastDigest enzyme<br /> +Alkaline phosphatase<br /> +</p> + <p className="indent">1. Add 1μg plasmid DNA, 1μl FastDigest enzyme, 2μl 10× FastDigest or 10× FastDigest<br /> +2. Green Buffer and ddH2O to 20μl into an PCR tube.<br /> +3. Put the tube into the oven at 37℃ for 1h to 1.5h.<br /> +4. Add 1μl Alkaline phosphatase.<br /> +5. Put the tube into the oven at 37℃ for 0.5h.<br /> +6. Stop the reaction by placing the tube at -4°C for 5 to 10 minutes.<br /> +7. Agarose Gel Electrophoresis.<br /> +8. Store the tube into the refrigerator at -20℃.<br /> +</p> </div> </Element>