From 3baf544940fd111dd4d130e97874366185559e68 Mon Sep 17 00:00:00 2001
From: Yuhao Lin <lyh2001213@163.com>
Date: Wed, 2 Oct 2024 05:44:55 +0000
Subject: [PATCH] =?UTF-8?q?=E4=BF=AE=E6=94=B9section2=E5=86=85=E5=AE=B9?=
=?UTF-8?q?=E4=B8=BADouble=20Enzyme=20DNA=20Digestion&=20Agarose=20Gel=20E?=
=?UTF-8?q?lectrophoresis?=
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---
src/contents/experiments.tsx | 22 ++++++++++++++++++----
1 file changed, 18 insertions(+), 4 deletions(-)
diff --git a/src/contents/experiments.tsx b/src/contents/experiments.tsx
index 53c4ca1..7fe5ad2 100644
--- a/src/contents/experiments.tsx
+++ b/src/contents/experiments.tsx
@@ -230,12 +230,26 @@ LB selective medium (Chloramphenicol)<br />
onMouseEnter={() => handleMouseEnter('section2')}
style={{ display: visibleSection === 'section2' ? 'block' : 'none' }}
>
- <h2>LB medium preparation</h2>
+ <h2>Double Enzyme DNA Digestion& Agarose Gel Electrophoresis</h2>
<p>Content for section 2.</p>
<div className="rounded-border">
- <h4 className="center-text">LB medium preparation</h4>
- <p className="indent">las ijffs aiskfd fskj iiwls asd.aass ffas awssd awus iisal fask.aisisad ksjdfkaf iwjasifjakdshf wijdfalksjf wiksjkfjksalhf, gsahfjhgejkfh uhaejkfh sjdihgfuqiw jh sjiafhjsaj fh asd.</p>
- <p className="indent">las ijffs aiskfd fskj iiwls asd.aass ffas awssd awus iisal fask.aisisad ksjdfkaf iwjasifjakdshf wijdfalksjf wiksjkfjksalhf, gsahfjhgejkfh uhaejkfh sjdihgfuqiw jh sjiafhjsaj fh asd.</p>
+ <h4 className="center-text">Double Enzyme DNA Digestion& Agarose Gel Electrophoresis</h4>
+ <p className="indent">（1）Material<br />
+Plasmid DNA<br />
+10× FastDigest or 10× FastDigest Green Buffer<br />
+Double Distillation Water (ddH2O)<br />
+FastDigest enzyme<br />
+Alkaline phosphatase<br />
+</p>
+ <p className="indent">1. Add 1μg plasmid DNA, 1μl FastDigest enzyme, 2μl 10× FastDigest or 10× FastDigest<br />
+2. Green Buffer and ddH2O to 20μl into an PCR tube.<br />
+3. Put the tube into the oven at 37℃ for 1h to 1.5h.<br />
+4. Add 1μl Alkaline phosphatase.<br />
+5. Put the tube into the oven at 37℃ for 0.5h.<br />
+6. Stop the reaction by placing the tube at -4°C for 5 to 10 minutes.<br />
+7. Agarose Gel Electrophoresis.<br />
+8. Store the tube into the refrigerator at -20℃.<br />
+</p>
</div>
</Element>
--
GitLab