<div>2. The target gene was implanted into E.coli DH5α/ PSB1A3-MRFP</div>
<div>2. The target gene was implanted into E.coli DH5α/ pSB1A3-MRFP</div>
<div>3. The plasmid was extracted, DNA was amplified by PCR, and double digestion and gel recovery experiments were performed</div>
<div>4. Competent cells were prepared, and the engineered vector PSB1A3-target fragment was transformed into the expression host E.coli BL21</div>
<div>4. Competent cells were prepared, and the engineered vector pSB1A3-target fragment was transformed into the expression host E.coli BL21</div>
<div>5. Molecular cloning and screening were performed to obtain positive clones, and the labeled DH5α/pSB1A3 target fragment colonies were selected for colony PCR with Tap enzyme.</div>