Skip to content
Snippets Groups Projects
Commit a1d9527f authored by Zonghan Liu's avatar Zonghan Liu
Browse files

Update wiki/pages/engineering.html

parent 1e98fcf6
No related branches found
No related tags found
No related merge requests found
Pipeline #99334 passed
......@@ -201,9 +201,9 @@
<div class="title2">
<div style="font-weight: bold;">Construction</div>
<div>1. Obtain the target gene - Tyrosinase</div>
<div>2. The target gene was implanted into E.coli DH5α/ PSB1A3-MRFP</div>
<div>2. The target gene was implanted into E.coli DH5α/ pSB1A3-MRFP</div>
<div>3. The plasmid was extracted, DNA was amplified by PCR, and double digestion and gel recovery experiments were performed</div>
<div>4. Competent cells were prepared, and the engineered vector PSB1A3-target fragment was transformed into the expression host E.coli BL21</div>
<div>4. Competent cells were prepared, and the engineered vector pSB1A3-target fragment was transformed into the expression host E.coli BL21</div>
<div>5. Molecular cloning and screening were performed to obtain positive clones, and the labeled DH5α/pSB1A3 target fragment colonies were selected for colony PCR with Tap enzyme.</div>
</div>
<div class="title2">
......
0% Loading or .
You are about to add 0 people to the discussion. Proceed with caution.
Finish editing this message first!
Please register or to comment