diff --git a/wiki/pages/engineering.html b/wiki/pages/engineering.html index e506e108812f7d2f590db9e628293fcecdd53460..5f5f7e2da146d3945d3a2fbf08614f3517c2bba4 100755 --- a/wiki/pages/engineering.html +++ b/wiki/pages/engineering.html @@ -201,9 +201,9 @@ <div class="title2"> <div style="font-weight: bold;">Construction</div> <div>1. Obtain the target gene - Tyrosinase</div> - <div>2. The target gene was implanted into E.coli DH5α/ PSB1A3-MRFP</div> + <div>2. The target gene was implanted into E.coli DH5α/ pSB1A3-MRFP</div> <div>3. The plasmid was extracted, DNA was amplified by PCR, and double digestion and gel recovery experiments were performed</div> - <div>4. Competent cells were prepared, and the engineered vector PSB1A3-target fragment was transformed into the expression host E.coli BL21</div> + <div>4. Competent cells were prepared, and the engineered vector pSB1A3-target fragment was transformed into the expression host E.coli BL21</div> <div>5. Molecular cloning and screening were performed to obtain positive clones, and the labeled DH5α/pSB1A3 target fragment colonies were selected for colony PCR with Tap enzyme.</div> </div> <div class="title2">