From a1d9527f4a2017a798ea5b603c67bb54e7d2d666 Mon Sep 17 00:00:00 2001 From: Zonghan Liu <lzh026677@outlook.com> Date: Sun, 9 Oct 2022 06:25:59 +0000 Subject: [PATCH] Update wiki/pages/engineering.html --- wiki/pages/engineering.html | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/wiki/pages/engineering.html b/wiki/pages/engineering.html index e506e10..5f5f7e2 100755 --- a/wiki/pages/engineering.html +++ b/wiki/pages/engineering.html @@ -201,9 +201,9 @@ <div class="title2"> <div style="font-weight: bold;">Construction</div> <div>1. Obtain the target gene - Tyrosinase</div> - <div>2. The target gene was implanted into E.coli DH5α/ PSB1A3-MRFP</div> + <div>2. The target gene was implanted into E.coli DH5α/ pSB1A3-MRFP</div> <div>3. The plasmid was extracted, DNA was amplified by PCR, and double digestion and gel recovery experiments were performed</div> - <div>4. Competent cells were prepared, and the engineered vector PSB1A3-target fragment was transformed into the expression host E.coli BL21</div> + <div>4. Competent cells were prepared, and the engineered vector pSB1A3-target fragment was transformed into the expression host E.coli BL21</div> <div>5. Molecular cloning and screening were performed to obtain positive clones, and the labeled DH5α/pSB1A3 target fragment colonies were selected for colony PCR with Tap enzyme.</div> </div> <div class="title2"> -- GitLab