<p>We have tested the functionality of the designed muscone molecular switch in <i>Saccharomyces cerevisiae</i> using the GFP reporter gene intensity. Additionally, we have reduced background noise from wild-type strains by knocking out the original mating pathway receptors in <i>Saccharomyces cerevisiae</i>.</p>
<p>We have tested the functionality of the designed muscone molecular switch in <i>Saccharomyces cerevisiae</i> using the GFP reporter gene intensity. Additionally, we have reduced background noise from wild-type strains by knocking out the original mating pathway receptors in <i>Saccharomyces cerevisiae</i>.</p>
<p>For more information, please refer to <ahref="https://2024.igem.wiki/tsinghua/therapy-system"style="color: #FF5151">Therapy system</a>.</p>
<p>For more information, please refer to <ahref="https://2024.igem.wiki/tsinghua/therapy-system"style="color: #FF5151">Therapy system</a>.</p>
<h4>Communication with relevant social stakeholders</h4>
<h4>Communication with relevant social stakeholders</h4>
<p>To assess the potential for further applications of the muscone molecular switch in <i>Saccharomyces cerevisiae<i>, we communicated with Bluepha Company, which specializes in developing new microbial fermentation materials through synthetic biology. Bluepha Company's feedback indicated that the muscone molecular switch in our modified <i>Saccharomyces cerevisiae</i> could serve as an alternative to the traditional methanol promoter used in Pichia pastoris fermentation systems. Muscone offers cost-effectiveness and higher safety compared to methanol, making it an attractive option for the design of innovative fermentation processes in <i>Saccharomyces cerevisiae</i>.</p>
<p>To assess the potential for further applications of the muscone molecular switch in <i>Saccharomyces cerevisiae</i>, we communicated with Bluepha Company, which specializes in developing new microbial fermentation materials through synthetic biology. Bluepha Company's feedback indicated that the muscone molecular switch in our modified <i>Saccharomyces cerevisiae</i> could serve as an alternative to the traditional methanol promoter used in Pichia pastoris fermentation systems. Muscone offers cost-effectiveness and higher safety compared to methanol, making it an attractive option for the design of innovative fermentation processes in <i>Saccharomyces cerevisiae</i>.</p>
<p>Shortcoming: Background signal noise still exists; There are significant differences in baseline expression between different strains.</p>
<p>Shortcoming: Background signal noise still exists; There are significant differences in baseline expression between different strains.</p>
<p>Solution: Take further measures to modify the yeast genome to eliminate background noise; Introduce the musk ketone molecular switch gene into the yeast genome to avoid the impact of plasmid cloning variations, and screen for superior yeast strains for cultivation.</p>
<p>Solution: Take further measures to modify the yeast genome to eliminate background noise; Introduce the musk ketone molecular switch gene into the yeast genome to avoid the impact of plasmid cloning variations, and screen for superior yeast strains for cultivation.</p>
