From 77caafe71fd967b76f227841d73686a79c64e4d5 Mon Sep 17 00:00:00 2001
From: HouTeng Chan <ht-chen21@mails.tsinghua.edu.cn>
Date: Tue, 1 Oct 2024 21:26:54 +0000
Subject: [PATCH] Update file proof-of-concept.html
---
wiki/pages/proof-of-concept.html | 2 +-
1 file changed, 1 insertion(+), 1 deletion(-)
diff --git a/wiki/pages/proof-of-concept.html b/wiki/pages/proof-of-concept.html
index ed96f4c9..9e42297e 100644
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<p>We have tested the functionality of the designed muscone molecular switch in <i>Saccharomyces cerevisiae</i> using the GFP reporter gene intensity. Additionally, we have reduced background noise from wild-type strains by knocking out the original mating pathway receptors in <i>Saccharomyces cerevisiae</i>.</p>
<p>For more information, please refer to <a href="https://2024.igem.wiki/tsinghua/therapy-system" style="color: #FF5151" >Therapy system</a>.</p>
<h4>Communication with relevant social stakeholders</h4>
- <p>To assess the potential for further applications of the muscone molecular switch in <i>Saccharomyces cerevisiae<i>, we communicated with Bluepha Company, which specializes in developing new microbial fermentation materials through synthetic biology. Bluepha Company's feedback indicated that the muscone molecular switch in our modified <i>Saccharomyces cerevisiae</i> could serve as an alternative to the traditional methanol promoter used in Pichia pastoris fermentation systems. Muscone offers cost-effectiveness and higher safety compared to methanol, making it an attractive option for the design of innovative fermentation processes in <i>Saccharomyces cerevisiae</i>.</p>
+ <p>To assess the potential for further applications of the muscone molecular switch in <i>Saccharomyces cerevisiae</i>, we communicated with Bluepha Company, which specializes in developing new microbial fermentation materials through synthetic biology. Bluepha Company's feedback indicated that the muscone molecular switch in our modified <i>Saccharomyces cerevisiae</i> could serve as an alternative to the traditional methanol promoter used in Pichia pastoris fermentation systems. Muscone offers cost-effectiveness and higher safety compared to methanol, making it an attractive option for the design of innovative fermentation processes in <i>Saccharomyces cerevisiae</i>.</p>
<p>Shortcoming: Background signal noise still exists; There are significant differences in baseline expression between different strains.</p>
<p>Solution: Take further measures to modify the yeast genome to eliminate background noise; Introduce the musk ketone molecular switch gene into the yeast genome to avoid the impact of plasmid cloning variations, and screen for superior yeast strains for cultivation.</p>
<h3>Proof of secretion of lactate</h3>
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