<br><br>To add or change protein tags, we designed PCR primers to clone SUMO fragment from pYSW-2(pala-SUMO) (gifted by Gan Lab), and GST fragment from pBAD AQ_GST (See our <astyle="font-family: 'Montserrat';color:#e1a965;text-decoration:underline dotted #e1a965;text-align:right;font-size:inherit;font-weight: inherit;"href="{{ url_for('pages', page='parts')}}">
Parts</a>). To change promoters, we would clone genes of LAP and HSP60 and insert them into the backbone of pBAD AQ_GST plasmid (pBAD). See all of the primers in the Experiment.
Parts page</a>). To change promoters, we would clone genes of LAP and HSP60 and insert them into the backbone of pBAD AQ_GST plasmid (pBAD). See all of the primers in the Experiment.
<br><br>We conducted reverse PCR to linearize the vectors and PCR to amplify the fragments. After linearization, we would ligate the PCR products to construct the plasmids by homologous recombination.