From fa6804b1f06ae4cd072cdfe9def2506bb95a16e0 Mon Sep 17 00:00:00 2001
From: raven233 <123@1234.com>
Date: Wed, 2 Oct 2024 21:52:21 +0800
Subject: [PATCH] 1111
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wiki/pages/engineering.html | 2 +-
1 file changed, 1 insertion(+), 1 deletion(-)
diff --git a/wiki/pages/engineering.html b/wiki/pages/engineering.html
index ae34d96..47ae4f4 100644
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+++ b/wiki/pages/engineering.html
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<p><br>
<b>â— Build</b>
<br> <br>To add or change protein tags, we designed PCR primers to clone SUMO fragment from pYSW-2(pala-SUMO) (gifted by Gan Lab), and GST fragment from pBAD AQ_GST (See our <a style="font-family: 'Montserrat';color:#e1a965;text-decoration:underline dotted #e1a965;text-align:right;font-size:inherit;font-weight: inherit;" href="{{ url_for('pages', page='parts')}}">
- Parts</a>). To change promoters, we would clone genes of LAP and HSP60 and insert them into the backbone of pBAD AQ_GST plasmid (pBAD). See all of the primers in the Experiment.
+ Parts page</a>). To change promoters, we would clone genes of LAP and HSP60 and insert them into the backbone of pBAD AQ_GST plasmid (pBAD). See all of the primers in the Experiment.
<br> <br>We conducted reverse PCR to linearize the vectors and PCR to amplify the fragments. After linearization, we would ligate the PCR products to construct the plasmids by homologous recombination.
</p>
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