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2024 Competition
UCAS-China
Commits
a64373df
Commit
a64373df
authored
4 months ago
by
Xujinsheng
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a64373df
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@@ -88,10 +88,6 @@
</center>
<h2
id=
'what-kinds-of-situations-are-lantern-similar-devices-suitable-for'
>
What kinds of situations are
LANTERN-similar devices suitable for?
</h2>
<p>
Synthetic biology is an engineering discipline — there is a desire to build things that do not yet
exist (Church et al., 2014). We reflect on the essence in the realm of
"
engineering
"
, seeking
modular methods for gene circuit synthesis. Drawing inspiration from digital circuits, we completed the design of
LANTERN, a programmable logic framework.
</p>
<p>
Through LANTERN, we can generate 16 different output mappings from two input molecules. In LANTERN we use IPTG and
rha as input small molecules and GFP fluorescence as the output signal. This represents a new paradigm—— elements
like LANTERN should be able to evolve to accept any molecule as input and produce any signal as output. Based on
...
...
@@ -205,10 +201,6 @@
style=
"zoom: 60%;"
>
<div
style=
"color: #666666; padding: 2px;"
>
Fig 3. five phases divided by two intermediate metabolites
</div>
</center>
<p>
LANTERN
'
s simple display also gives it the potential for everyday applications, such as home food fermentation,
like making pickles.
</p>
<p>
we can use Lantern to program the detection of any harmful substances, thereby achieving the effect of simply
detecting harmful substances.
</p>
<h3
id=
'synthetic-circuits-integrating-logic'
>
Synthetic circuits integrating logic
</h3>
<p>
Since the rise of synthetic biology, scientists have been searching for a strategy for efficiently assembling
synthetic genetic circuits to achieve Boolean logic functions with stable DNA-encoded memory of events(Siuti et al.,
...
...
@@ -218,10 +210,10 @@
biological memory storage systems.
</p>
<h4
id=
'synthetic-biology-system-stability-regulation-module'
>
Synthetic Biology System Stability Regulation Module
</h4>
<p>
We hope that LANTERN-
similar
devices can contribute to the synthetic biology community. We soon realized that they
<p>
We hope that LANTERN-
like
devices can contribute to the synthetic biology community. We soon realized that they
could play a role in preventing mutations and detecting the normal operation of genetic pathways.
</p>
<p>
As an example, we can look at
the UCAS-China 2023 project
"
NOX: Neo-quOrum sensing-based Xpression biosensor
platform
.
"
NOX provides a highly compatible and robust platform with impressive performance. Chimeric receptors
<p>
Take
the UCAS-China 2023 project
"
NOX: Neo-quOrum sensing-based Xpression biosensor
platform." as an
example
.
"
NOX provides a highly compatible and robust platform with impressive performance. Chimeric receptors
are assembled for optimal compatibility, and the orthogonal quorum sensing module is responsible for luminescence,
optimized through modeling (see:
<a
href=
'https://2023.igem.wiki/ucas-china/'
>
https://2023.igem.wiki/ucas-china/
</a>
). In the experimental design, NOX
...
...
@@ -236,9 +228,10 @@
<h2
id=
'drylab'
>
Drylab
</h2>
<h3
id=
'software'
>
Software
</h3>
<p>
To support our project's pre-research phase and give visualization about how our circuits work, we developed two
tools: (1) a circuit visualization website
, illustrating the functionality of our genetic circuits and making the
tools: (1) a circuit visualization website, illustrating the functionality of our genetic circuits and making the
programmable process of LANTERN easier; (2) Ask Lantern, a natural language processing model for searching Biobricks
(See Software for more details), aimed at reducing the pre-research time finding suitable Biobricks from the massive
(See
<a
href=
'https://2024.igem.wiki/ucas-china/software'
>
Software
</a>
for more details), aimed at reducing the
pre-research time finding suitable Biobricks from the massive
Biobricks database.
</p>
<p>
For project pre-research, we developed a natural language query program for the Biobricks database called Ask
Lantern (See
<a
href=
'https://2024.igem.wiki/ucas-china/software'
>
Software
</a>
for more details), aimed at reducing
...
...
@@ -255,8 +248,7 @@
<p>
Our model comprises four components: 1) ensuring functionality of constant promoter, 2) characteristics of the
logic AND Gate, 3) Demonstrating the inhibition induced by CRISPRi, and 4) estimating the time consumption of the
boolean
logic circuit system. Modeling is employed to predict outcomes and support the verification of LANTERN, as
illustrated in the four components shown in the figure.
</p>
logic circuit system.
</p>
<center>
<img
src=
"https://static.igem.wiki/teams/5276/model/abstractnew2.webp"
alt=
"orthogonality-design-1-new.webp"
style=
"zoom: 60%;"
>
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