<p>At present, 5-ALA is mainly synthesized by chemical synthesis and microbial fermentation. The study of chemical synthesis began in the 50s of the 20th century, and was most active in the 90s. The researchers synthesized 5-ALA [9] using raw materials such as hippuric acid, succinic acid, tetrahydrofurfuramine, and levulinic acid. However, the chemical synthesis method has the disadvantages of high price, difficult to obtain, high toxicity, low production yield and harsh reaction conditions, and the synthetic products contain impurities that are difficult to determine, which may cause potential harm to agricultural and forestry [8]. <strong>In this project, <i>E. coli</i> was used as the chassis to screen strains that can produce stable and high yields of 5-ALA through enzyme modification, CRISPR transposition, and microfluidic high-throughput screening</strong>. Finally, the efficient and sustainable production of 5-ALA will be realized, thereby reducing the cost of biopesticides and contributing to the alleviation of the global food crisis. </p>
<p>At present, 5-ALA is mainly synthesized by chemical synthesis and microbial fermentation. The study of chemical synthesis began in the 50s of the 20th century, and was most active in the 90s. The researchers synthesized 5-ALA [9] using raw materials such as hippuric acid, succinic acid, tetrahydrofurfuramine, and levulinic acid. However, the chemical synthesis method has the disadvantages of high price, difficult to obtain, high toxicity, low production yield and harsh reaction conditions, and the synthetic products contain impurities that are difficult to determine, which may cause potential harm to agricultural and forestry [8]. <strong>In this project, <i>E. coli</i> was used as the chassis to screen strains that can produce stable and high yields of 5-ALA through enzyme modification, CRISPR-associated transposons (CASTs) system, and microfluidic high-throughput screening</strong>. Finally, the efficient and sustainable production of 5-ALA will be realized, thereby reducing the cost of biopesticides and contributing to the alleviation of the global food crisis. </p>
