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Commit 814b1fcd authored by Natasha Nunez's avatar Natasha Nunez
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......@@ -48,6 +48,7 @@ This kit provides precise and visual tutorials on the most common and essential
Here is an example of one of the videos that have been uploaded!
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<h4> <b>Video 1.</b> Natronaut's iGEM Survival Kit Episode 1 - How to Prepare Liquid Cultures</h4>
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......@@ -60,7 +61,11 @@ Here is an example of one of the videos that have been uploaded!
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<figcaption style="font-size: 14px; color: #333; text-align: justify; width: 100%; margin: 0;">
In this video, Natronaut explains how to prepare liquid cultures of bacterial colonies.
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For wet laboratories, the following videos have been posted: how to do liquid cultures from bacterial growth on plates, how to perform plasmid isolation by miniprep, and how to carry out bacterial transformation (in this case, the protocol focused on Vibrio natriegens transformation). Although only the first three have been posted, many more are currently being edited and will be uploaded to Natronaut’s Instagram page soon. The videos that are currently planned include: how to do gene fragment amplification, how to perform plasmid linearisation, DpnI enzymatic cleanup, gel electrophoresis, Gibson assembly, transformation, colony Polymerase Chain Reaction (PCR), and how to prepare samples for sequencing.
......@@ -68,6 +73,7 @@ The second part of the series focuses on dry-lab methods; in this section, we ai
For this series, currently, only one video has been posted: how to visualise DNA sequences. This video (as well as all videos that will follow) is first introduced by a short recap lecture covering all concepts, such as the central dogma of molecular biology and synthetic DNA sequences.
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<h4> <b>Video 2.</b> Natronaut's iGEM Survival Kit Episode 4 - How to visualise DNA Sequences</h4>
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......@@ -80,7 +86,11 @@ For this series, currently, only one video has been posted: how to visualise DNA
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<figcaption style="font-size: 14px; color: #333; text-align: justify; width: 100%; margin: 0;">
In this video, Natronaut explains how to visualise DNA sequences using Addgene, GenBank, UniProt, and a reverse translation tool.
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Similarly to the wet laboratory videos, there are many other videos which are planned: how to design primers on SnapGene, how to simulate Gibson assembly and gel electrophoresis, how to design sequencing primers, how to work with operons and finally how to analyse sequencing results.
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