For wet laboratories, the following videos have been posted: how to do liquid cultures from bacterial growth on plates, how to perform plasmid isolation by miniprep, and how to carry out bacterial transformation (in this case, the protocol focused on Vibrio natriegens transformation). Although only the first three have been posted, many more are currently being edited and will be uploaded to Natronaut’s Instagram page soon. The videos that are currently planned include: how to do gene fragment amplification, how to perform plasmid linearisation, DpnI enzymatic cleanup, gel electrophoresis, Gibson assembly, transformation, colony Polymerase Chain Reaction (PCR), and how to prepare samples for sequencing.
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For this series, currently, only one video has been posted: how to visualise DNA sequences. This video (as well as all videos that will follow) is first introduced by a short recap lecture covering all concepts, such as the central dogma of molecular biology and synthetic DNA sequences.
**VIDEO TO BE ADDED HERE: FOUND ON IGEM24>WIKI>EDUCATION_VID2_IGSK**
<h4><b>Video 2.</b> Natronaut's iGEM Survival Kit Episode 4 - How to visualise DNA Sequences</h4>
Similarly to the wet laboratory videos, there are many other videos which are planned: how to design primers on SnapGene, how to simulate Gibson assembly and gel electrophoresis, how to design sequencing primers, how to work with operons and finally how to analyse sequencing results.
