Our team has registered new parts in the iGEM registry, aiming to facilitate research using the ANRA pathway with or without <i>Vibrio Natriegens</i>. ADD DETAILS OF THE PARTS THEMSELVES: TYPE EG. BASIC, COMPOSITE WITH NUMBER. By the registry of these parts, we aim to further expand the accessibility of such parts for future iGEM teams that wish to work with the assimilatory nitrate reduction to ammonium (ANRA) pathway without or with <i>Vibrio Natriegens</i> as a chassis organism. Parts also come with characterization which can aid and provide a starting point for future teams. Furthermore, we wish for such future iGEM teams to continue to characterize and build upon these newly registered iGEM parts with their own findings, truly setting the foundations for collaboration.
DO A TABLE WITH OUR CATEGORIES OF PARTS AND THEIR NUMBERS
For detailed information about Natronaut's parts please refer to the Engineering and Parts pages. LINK
Natronaut is proud to introduce several new BioBrick Parts to the iGEM Parts Registry. The iGEM Registry is the best way to contribute to future iGEM teams, who can find and utilize well-characterized parts with increased ease. Our registered parts consist of six new basic parts and the composite part they contribute to. Below is a brief description of each part we contributed, including their roles in the full Assimilatory Nitrate Reduction to Ammonium (ANRA) pathway and other potential applications:
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<li><b>Periplasmic Binding Protein (nasF) - BBa_K5306000</b><br>
The periplasmic binding protein binds to extracellular nitrates with high affinity, starting the uptake process necessary for the functioning of the ANRA pathway and overall nitrate capture.</li>
<li><b>Homodimeric Transmembrane Protein (nasE) - BBa_K5306001</b><br>
This transmembrane protein facilitates the uptake of nitrate through the lipid bilayer, acting as a vital link in the transport of nitrates into the cell.</li>
<li><b>Dimeric ATP-Binding Protein (nasD) - BBa_K5306002</b><br>
The nasD part encodes a dimeric ATP-binding protein that hydrolyzes ATP. This protein powers the transport processes necessary for the uptake of nitrates, ensuring that the pathway operates efficiently under varying conditions.</li>
This enzyme is responsible for catalyzing the conversion of nitrate (NO₃⁻) to nitrite (NO₂⁻) once it enters the bacterial cell. Nitrate reductase is NADH-dependent and consists of a large catalytic subunit containing the active site for reduction and a smaller NADH oxidoreductase subunit, facilitating electron transfer.</li>
The nitrite reductase enzyme converts nitrites (NO₂⁻) into ammonium (NH₄⁺). As a cytochrome enzyme, it employs various heme groups to facilitate the reduction process, ensuring efficient conversion. The sequence encoding nasB also includes a 6x His tag, enabling protein localization.</li>
This composite part encodes the three primary enzymes involved in the ANRA pathway: the transporter (ABC enzyme), nitrate reductase, and nitrite reductase. By integrating these components, the ANRA pathway enables the efficient conversion of nitrates to ammonium.</li>
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These parts not only play key roles both individually and as part of the ANRA pathway but also facilitate the collaborative research and development that is central to the iGEM community. These parts can be utilized independently or in combination to engineer innovative solutions.
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For more detailed information about Natronaut's parts, please refer to the Engineering and Parts pages. <!-- Insert your LINK here -->
