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Commit cdcf89ed authored by Liliana Sanfilippo's avatar Liliana Sanfilippo
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Abbildungsnummern einfügen

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...@@ -304,7 +304,7 @@ export function Description() { ...@@ -304,7 +304,7 @@ export function Description() {
<OneFigure <OneFigure
pic1="https://ars.els-cdn.com/content/image/1-s2.0-S1773224724002156-gr3_lrg.jpg" pic1="https://ars.els-cdn.com/content/image/1-s2.0-S1773224724002156-gr3_lrg.jpg"
alt1="Aufnahme LNP" alt1="Aufnahme LNP"
num={8} num={7}
description={<span> Endosomal escape vs degradation of LNP cargo at endocytosis <SupScrollLink label="4"/>.</span>} description={<span> Endosomal escape vs degradation of LNP cargo at endocytosis <SupScrollLink label="4"/>.</span>}
/> />
</div> </div>
...@@ -333,7 +333,7 @@ export function Description() { ...@@ -333,7 +333,7 @@ export function Description() {
pic1="https://static.igem.wiki/teams/5247/delivery/big-plan-inhalation-teil-del.webp" pic1="https://static.igem.wiki/teams/5247/delivery/big-plan-inhalation-teil-del.webp"
alt1="" alt1=""
description="Schematic representation our LNP-based drug delivery system." description="Schematic representation our LNP-based drug delivery system."
num="9" num="8"
/> />
</div> </div>
<p>To evaluate the <strong>delivery efficiency</strong>, we transfected HEK293 and CFBE41o- cells using fluorescent cargo and quantified the results through flow cytometry analysis. We also ensured that AirBuddy meets the necessary standards for safety and efficacy since we conducted extensive <a onClick={() => goToPageAndScroll ('In-Depth Characterization of LNPsH', '/materials-methods')}> characterization of the LNPs </a>using physicochemical techniques such as Zeta potential analysis, Dynamic Light Scattering (DLS), Scanning Electron Microscopy (SEM), and Cryogenic Electron Microscopy (cryo-EM). These methods confirmed the stability and optimal size distribution of the nanoparticles. Furthermore, <strong>cytotoxicity assessments</strong> including MTT and proliferation assays demonstrated that our LNPs are biocompatible despite the incorporation of <a onClick={() => goToPageWithTabAndCollapsible({tabId:'tab-delivery', path: '/engineering', collapseId: "Col1"})}>PEG</a> and other ambivalent components. These findings reinforce AirBuddy's potential as a safe and effective tool for pulmonary delivery, with broad implications for gene therapies targeting lung diseases.</p> <p>To evaluate the <strong>delivery efficiency</strong>, we transfected HEK293 and CFBE41o- cells using fluorescent cargo and quantified the results through flow cytometry analysis. We also ensured that AirBuddy meets the necessary standards for safety and efficacy since we conducted extensive <a onClick={() => goToPageAndScroll ('In-Depth Characterization of LNPsH', '/materials-methods')}> characterization of the LNPs </a>using physicochemical techniques such as Zeta potential analysis, Dynamic Light Scattering (DLS), Scanning Electron Microscopy (SEM), and Cryogenic Electron Microscopy (cryo-EM). These methods confirmed the stability and optimal size distribution of the nanoparticles. Furthermore, <strong>cytotoxicity assessments</strong> including MTT and proliferation assays demonstrated that our LNPs are biocompatible despite the incorporation of <a onClick={() => goToPageWithTabAndCollapsible({tabId:'tab-delivery', path: '/engineering', collapseId: "Col1"})}>PEG</a> and other ambivalent components. These findings reinforce AirBuddy's potential as a safe and effective tool for pulmonary delivery, with broad implications for gene therapies targeting lung diseases.</p>
...@@ -355,7 +355,7 @@ export function Description() { ...@@ -355,7 +355,7 @@ export function Description() {
<p>Ultimately, our vision is to create a therapeutic approach that not only offers a cure that is safe and efficient but also maximizes convenience for the patient. With an easy-to-use inhaler, patients could administer their treatment with minimal disruption to their daily lives, inhaling the gene therapy in just a few breaths, leaving the rest of the process to the science we've built into PreCyse. By reducing the frequency of administration and simplifying the delivery method, we aim to make gene therapy for Cystic Fibrosis both accessible and practical for patients around the world. </p> <p>Ultimately, our vision is to create a therapeutic approach that not only offers a cure that is safe and efficient but also maximizes convenience for the patient. With an easy-to-use inhaler, patients could administer their treatment with minimal disruption to their daily lives, inhaling the gene therapy in just a few breaths, leaving the rest of the process to the science we've built into PreCyse. By reducing the frequency of administration and simplifying the delivery method, we aim to make gene therapy for Cystic Fibrosis both accessible and practical for patients around the world. </p>
<OneFigure <OneFigure
pic1="https://static.igem.wiki/teams/5247/delivery/big-plan-inhalation-del-mech.webp" pic1="https://static.igem.wiki/teams/5247/delivery/big-plan-inhalation-del-mech.webp"
num={10} num={9}
description="Illustration of our path from final product to prime editing in lung epithelial cells." description="Illustration of our path from final product to prime editing in lung epithelial cells."
alt1="Illustration of our path from final product to prime editing in lung epithelial cells." alt1="Illustration of our path from final product to prime editing in lung epithelial cells."
/> />
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