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Commit 6700e1a3 authored by Kathleen Susat's avatar Kathleen Susat
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pics mech results

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...@@ -6,7 +6,7 @@ import { useTabNavigation } from "../utils/TabNavigation"; ...@@ -6,7 +6,7 @@ import { useTabNavigation } from "../utils/TabNavigation";
import { H5 } from "../components/Headings"; import { H5 } from "../components/Headings";
import { useNavigation } from "../utils"; import { useNavigation } from "../utils";
import { DownloadLink } from "../components/Buttons"; import { DownloadLink } from "../components/Buttons";
import { TwoHorizontal } from "../components/Figures"; import { TwoHorizontal, TwoVertical } from "../components/Figures";
export function Results() { export function Results() {
...@@ -65,27 +65,63 @@ export function Results() { ...@@ -65,27 +65,63 @@ export function Results() {
num={1} num={1}
pic1="https://static.igem.wiki/teams/5247/photos/facs-results-mechanism/se-nose.png" pic1="https://static.igem.wiki/teams/5247/photos/facs-results-mechanism/se-nose.png"
pic2="https://static.igem.wiki/teams/5247/photos/facs-results-mechanism/bild8.png" pic2="https://static.igem.wiki/teams/5247/photos/facs-results-mechanism/bild8.png"
/> />
<H4 text="pegRNA 2"/> <H4 text="pegRNA 2"/>
<H5 text="Workflow"/> <H5 text="Workflow"/>
<p>Cotransfection of pPEAR_CFTR and PE2 and also 1 of the 14 pegRNAs to compare the transfection efficiency of all of our designed pegRNAs.</p> <p>Cotransfection of pPEAR_CFTR and PE2 and also 1 of the 14 pegRNAs to compare the transfection efficiency of all of our designed pegRNAs.</p>
<H5 text="Conclusion"/> <H5 text="Conclusion"/>
<p>The pegRNAs lead to differing amounts of cells showing fluorescence, which, assuming comparable transfection efficiencies, indicates varying prime editing efficiency. The pegRNA7 showed the highest transfection efficiency (see Figure X).</p> <p>The pegRNAs lead to differing amounts of cells showing fluorescence, which, assuming comparable transfection efficiencies, indicates varying prime editing efficiency. The pegRNA7 showed the highest transfection efficiency (see Figure X).</p>
<div className="figure-wrapper">
<figure>
<img src="https://static.igem.wiki/teams/5247/photos/facs-results-mechanism/bild9.png" style={{height: "10%", width: "auto"}}/>
<figcaption> <b>Figure X</b>XXXXX</figcaption>
</figure>
</div>
<H4 text="pegRNA 3"/> <H4 text="pegRNA 3"/>
<H5 text="Workflow"/> <H5 text="Workflow"/>
<p>We tried to transfect CFBE41o- cells with pDAS12124-preedited, our internal positive control, to check if a transfection of this cell line is possible. Furthermore we tried to co transfect the CFBE41o- with pPEAR_CFTR, PE6c and pegRNA4.</p> <p>We tried to transfect CFBE41o- cells with pDAS12124-preedited, our internal positive control, to check if a transfection of this cell line is possible. Furthermore we tried to co transfect the CFBE41o- with pPEAR_CFTR, PE6c and pegRNA4.</p>
<H5 text="Conclusion"/> <H5 text="Conclusion"/>
<p>Transfection of CFBE41o- with pDAS12124-preedited was successful (see Figure X). After 24 hours a successful co transfection of pPEAR_CFTR with PE6c and pegRNA4 was visible, although the transfection efficiency was really bad (see Figure X).</p> <p>Transfection of CFBE41o- with pDAS12124-preedited was successful (see Figure X). After 24 hours a successful co transfection of pPEAR_CFTR with PE6c and pegRNA4 was visible, although the transfection efficiency was really bad (see Figure X).</p>
<TwoVertical
description="Microscopy results after 24h or 48h. Transfection of pDAS12124-preedited with lipofectamine 3000 was successfully done in CFBE41o- cell line and visible after 48h. CFBE41o- cell line was transfected with pDAS-IDT with Lipofectamine 3000 and afterwards with LNPs including PE6c and pegRNA4 and was after 24h fluorescence visible."
num={2}
pic1="https://static.igem.wiki/teams/5247/photos/facs-results-mechanism/bild10-1.png"
pic2="https://static.igem.wiki/teams/5247/photos/facs-results-mechanism/bild10-2.png"
/>
<div className="figure-wrapper">
<figure>
<img src="https://static.igem.wiki/teams/5247/photos/facs-results-mechanism/pe2-pe-co.png" style={{height: "10%", width: "auto"}}/>
<figcaption> <b>Figure X</b> XXXX</figcaption>
</figure>
</div>
<H4 text="pegRNA 4"/> <H4 text="pegRNA 4"/>
<H5 text="Workflow"/> <H5 text="Workflow"/>
<p>pCMV-PE2 was co transfected with pDAS12489 and pCMV-PE_CO-Mini was co transfected with pDAS12489 in HEK293 cell line.</p> <p>pCMV-PE2 was co transfected with pDAS12489 and pCMV-PE_CO-Mini was co transfected with pDAS12489 in HEK293 cell line.</p>
<H5 text="Conclusion"/> <H5 text="Conclusion"/>
<p>The FACS results show that transfection with pCMV-PE2 as the prime editing complex had editing efficiency of 52.90% when normalized on pDAS12124-preedited. When pCMV-PE_CO-Mini was used as a prime editing complex it had a transfection efficiency of 2.54% (see Figure X).</p> <p>The FACS results show that transfection with pCMV-PE2 as the prime editing complex had editing efficiency of 52.90% when normalized on pDAS12124-preedited. When pCMV-PE_CO-Mini was used as a prime editing complex it had a transfection efficiency of 2.54% (see Figure X).</p>
<TwoVertical
description="Microscopy results after 72h. ."
num={3}
pic1="https://static.igem.wiki/teams/5247/photos/facs-results-mechanism/bild11-1.png"
pic2="https://static.igem.wiki/teams/5247/photos/facs-results-mechanism/bild11-2.png"
/>
<H4 text="pegRNA 5"/> <H4 text="pegRNA 5"/>
<H5 text="Workflow"/> <H5 text="Workflow"/>
<p>We compared the 3 different Prime Editing complexes (pCMV-PE2, pCMV-PE2_CO-Mini & pCMV-PE6c) to check which one has the best transfection efficiency.</p> <p>We compared the 3 different Prime Editing complexes (pCMV-PE2, pCMV-PE2_CO-Mini & pCMV-PE6c) to check which one has the best transfection efficiency.</p>
<H5 text="Conclusion"/> <H5 text="Conclusion"/>
<p>The FACS measurement shows the fluorescence rate cells co-transfected with pDAS12489 and pCMV-PE6c as a prime editing complex. The editing efficiency off PE6c was by far the highest (81.88%) (see Figure X). The efficiency was 1.55 higher than the efficiency when pCMV-PE2 was used as prime editing complex (see Figure X).</p> <p>The FACS measurement shows the fluorescence rate cells co-transfected with pDAS12489 and pCMV-PE6c as a prime editing complex. The editing efficiency off PE6c was by far the highest (81.88%) (see Figure X). The efficiency was 1.55 higher than the efficiency when pCMV-PE2 was used as prime editing complex (see Figure X).</p>
<div className="figure-wrapper">
<figure>
<img src="https://static.igem.wiki/teams/5247/photos/facs-results-mechanism/bild13.png" style={{height: "10%", width: "auto"}}/>
<figcaption> <b>Figure X</b> XXXX</figcaption>
</figure>
</div>
<TwoHorizontal
description="XXXXXXXXX"
num={4}
pic1="https://static.igem.wiki/teams/5247/photos/facs-results-mechanism/pe2-pe-co-pe6c.png"
pic2="https://static.igem.wiki/teams/5247/photos/facs-results-mechanism/bild12.png"
/>
</Subesction > </Subesction >
<Subesction title="Delivery" id="Results3"> <Subesction title="Delivery" id="Results3">
<H4 text="RNA Synthesis"/> <H4 text="RNA Synthesis"/>
......
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