diff --git a/src/contents/results.tsx b/src/contents/results.tsx index e374a2ff81eb26f01a14260725d4f05bc3ef73e0..977411346c475819f9cb36a6f8098a3a3b255cdd 100644 --- a/src/contents/results.tsx +++ b/src/contents/results.tsx @@ -6,7 +6,7 @@ import { useTabNavigation } from "../utils/TabNavigation"; import { H5 } from "../components/Headings"; import { useNavigation } from "../utils"; import { DownloadLink } from "../components/Buttons"; -import { TwoHorizontal } from "../components/Figures"; +import { TwoHorizontal, TwoVertical } from "../components/Figures"; export function Results() { @@ -65,27 +65,63 @@ export function Results() { num={1} pic1="https://static.igem.wiki/teams/5247/photos/facs-results-mechanism/se-nose.png" pic2="https://static.igem.wiki/teams/5247/photos/facs-results-mechanism/bild8.png" - /> + /> <H4 text="pegRNA 2"/> <H5 text="Workflow"/> <p>Cotransfection of pPEAR_CFTR and PE2 and also 1 of the 14 pegRNAs to compare the transfection efficiency of all of our designed pegRNAs.</p> <H5 text="Conclusion"/> <p>The pegRNAs lead to differing amounts of cells showing fluorescence, which, assuming comparable transfection efficiencies, indicates varying prime editing efficiency. The pegRNA7 showed the highest transfection efficiency (see Figure X).</p> + <div className="figure-wrapper"> + <figure> + <img src="https://static.igem.wiki/teams/5247/photos/facs-results-mechanism/bild9.png" style={{height: "10%", width: "auto"}}/> + <figcaption> <b>Figure X</b>XXXXX</figcaption> + </figure> + </div> <H4 text="pegRNA 3"/> <H5 text="Workflow"/> <p>We tried to transfect CFBE41o- cells with pDAS12124-preedited, our internal positive control, to check if a transfection of this cell line is possible. Furthermore we tried to co transfect the CFBE41o- with pPEAR_CFTR, PE6c and pegRNA4.</p> <H5 text="Conclusion"/> <p>Transfection of CFBE41o- with pDAS12124-preedited was successful (see Figure X). After 24 hours a successful co transfection of pPEAR_CFTR with PE6c and pegRNA4 was visible, although the transfection efficiency was really bad (see Figure X).</p> + <TwoVertical + description="Microscopy results after 24h or 48h. Transfection of pDAS12124-preedited with lipofectamine 3000 was successfully done in CFBE41o- cell line and visible after 48h. CFBE41o- cell line was transfected with pDAS-IDT with Lipofectamine 3000 and afterwards with LNPs including PE6c and pegRNA4 and was after 24h fluorescence visible." + num={2} + pic1="https://static.igem.wiki/teams/5247/photos/facs-results-mechanism/bild10-1.png" + pic2="https://static.igem.wiki/teams/5247/photos/facs-results-mechanism/bild10-2.png" + /> + <div className="figure-wrapper"> + <figure> + <img src="https://static.igem.wiki/teams/5247/photos/facs-results-mechanism/pe2-pe-co.png" style={{height: "10%", width: "auto"}}/> + <figcaption> <b>Figure X</b> XXXX</figcaption> + </figure> + </div> <H4 text="pegRNA 4"/> <H5 text="Workflow"/> <p>pCMV-PE2 was co transfected with pDAS12489 and pCMV-PE_CO-Mini was co transfected with pDAS12489 in HEK293 cell line.</p> <H5 text="Conclusion"/> <p>The FACS results show that transfection with pCMV-PE2 as the prime editing complex had editing efficiency of 52.90% when normalized on pDAS12124-preedited. When pCMV-PE_CO-Mini was used as a prime editing complex it had a transfection efficiency of 2.54% (see Figure X).</p> + <TwoVertical + description="Microscopy results after 72h. ." + num={3} + pic1="https://static.igem.wiki/teams/5247/photos/facs-results-mechanism/bild11-1.png" + pic2="https://static.igem.wiki/teams/5247/photos/facs-results-mechanism/bild11-2.png" + /> <H4 text="pegRNA 5"/> <H5 text="Workflow"/> <p>We compared the 3 different Prime Editing complexes (pCMV-PE2, pCMV-PE2_CO-Mini & pCMV-PE6c) to check which one has the best transfection efficiency.</p> <H5 text="Conclusion"/> - <p>The FACS measurement shows the fluorescence rate cells co-transfected with pDAS12489 and pCMV-PE6c as a prime editing complex. The editing efficiency off PE6c was by far the highest (81.88%) (see Figure X). The efficiency was 1.55 higher than the efficiency when pCMV-PE2 was used as prime editing complex (see Figure X).</p> + <p>The FACS measurement shows the fluorescence rate cells co-transfected with pDAS12489 and pCMV-PE6c as a prime editing complex. The editing efficiency off PE6c was by far the highest (81.88%) (see Figure X). The efficiency was 1.55 higher than the efficiency when pCMV-PE2 was used as prime editing complex (see Figure X).</p> + <div className="figure-wrapper"> + <figure> + <img src="https://static.igem.wiki/teams/5247/photos/facs-results-mechanism/bild13.png" style={{height: "10%", width: "auto"}}/> + <figcaption> <b>Figure X</b> XXXX</figcaption> + </figure> + </div> + <TwoHorizontal + description="XXXXXXXXX" + num={4} + pic1="https://static.igem.wiki/teams/5247/photos/facs-results-mechanism/pe2-pe-co-pe6c.png" + pic2="https://static.igem.wiki/teams/5247/photos/facs-results-mechanism/bild12.png" + /> </Subesction > <Subesction title="Delivery" id="Results3"> <H4 text="RNA Synthesis"/>