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Commit 652c6d7e authored by Simon Klute's avatar Simon Klute
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<h2>Introduction</h2> <h2>Introduction</h2>
<p> <p>
This year, our team focused on working with the plant pathogen <i>Agrobacterium</i>. <i>Agrobacterium</i>, an <i>Alphaproteobacterium</i>, is This year, our team focused on working with the plant pathogen <i>Agrobacterium</i>. <i>Agrobacterium</i>, an Alphaproteobacterium, is
widely used for plant transformation due to its innate ability to insert a fragment known as transfer DNA (T-DNA) from widely used for plant transformation due to its innate ability to insert a fragment known as transfer DNA (T-DNA) from
its root-inducing plasmid (Ri-plasmid) into the plant host. The species <i>Agrobacterium rhizogenes</i> is renowned for causing its root-inducing plasmid (Ri-plasmid) into the plant host. The species <i>Agrobacterium rhizogenes</i> is renowned for causing
"hairy root disease" in plants, orchestrated by the expression of virulence genes (<i>vir</i> genes) located on the "hairy root disease" in plants, orchestrated by the expression of virulence genes (<i>vir</i> genes) located on the
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composite parts and transforming it into the <i>Agrobacterium rhizogenes</i> strains ARqua1 and K599. These composite parts composite parts and transforming it into the <i>Agrobacterium rhizogenes</i> strains ARqua1 and K599. These composite parts
included different versions of the transcriptional activator VirG, controlled by various inducible promoters. Our goal included different versions of the transcriptional activator VirG, controlled by various inducible promoters. Our goal
was to upregulate general virulence within a controlled timeframe in controlled laboratory conditions. To assess was to upregulate general virulence within a controlled timeframe in controlled laboratory conditions. To assess
transformation efficiency, both strains also carried the RUBY reporter construct , which contains the coding regions for transformation efficiency, both strains also carried the RUBY reporter construct, which contains the coding regions for
the cytochrome P450 enzyme CYP76AD1 , DOPA dioxygenase (DODA) and a glycosyltransferase, all separated self-cleaving 2A the cytochrome P450 enzyme CYP76AD1, DOPA dioxygenase (DODA) and a glycosyltransferase, all separated self-cleaving 2A
linker peptides. Successful integration of this construct into plant cells catalyzes the production of betalains, making linker peptides. Successful integration of this construct into plant cells catalyzes the production of betalains, making
them visible to the naked eye as red colouration (He et al., 2020). them visible to the naked eye as red colouration (He et al., 2020).
</p> </p>
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