From 652c6d7ed5146f27f295f06215f56afd089d8785 Mon Sep 17 00:00:00 2001 From: Simon Klute <klutes@students.uni-marbrug.de> Date: Tue, 12 Dec 2023 13:18:12 +0000 Subject: [PATCH] spelling --- wiki/pages/safety.html | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/wiki/pages/safety.html b/wiki/pages/safety.html index 95bf960..cb92317 100644 --- a/wiki/pages/safety.html +++ b/wiki/pages/safety.html @@ -12,7 +12,7 @@ <h2>Introduction</h2> <p> - This year, our team focused on working with the plant pathogen <i>Agrobacterium</i>. <i>Agrobacterium</i>, an <i>Alphaproteobacterium</i>, is + This year, our team focused on working with the plant pathogen <i>Agrobacterium</i>. <i>Agrobacterium</i>, an Alphaproteobacterium, is widely used for plant transformation due to its innate ability to insert a fragment known as transfer DNA (T-DNA) from its root-inducing plasmid (Ri-plasmid) into the plant host. The species <i>Agrobacterium rhizogenes</i> is renowned for causing "hairy root disease" in plants, orchestrated by the expression of virulence genes (<i>vir</i> genes) located on the @@ -68,8 +68,8 @@ composite parts and transforming it into the <i>Agrobacterium rhizogenes</i> strains ARqua1 and K599. These composite parts included different versions of the transcriptional activator VirG, controlled by various inducible promoters. Our goal was to upregulate general virulence within a controlled timeframe in controlled laboratory conditions. To assess - transformation efficiency, both strains also carried the RUBY reporter construct , which contains the coding regions for - the cytochrome P450 enzyme CYP76AD1 , DOPA dioxygenase (DODA) and a glycosyltransferase, all separated self-cleaving 2A + transformation efficiency, both strains also carried the RUBY reporter construct, which contains the coding regions for + the cytochrome P450 enzyme CYP76AD1, DOPA dioxygenase (DODA) and a glycosyltransferase, all separated self-cleaving 2A linker peptides. Successful integration of this construct into plant cells catalyzes the production of betalains, making them visible to the naked eye as red colouration (He et al., 2020). </p> -- GitLab