@@ -422,6 +421,7 @@ Flavin reductase (Fre) regenerates FAD to FADH2 using NAD(P)H.</span>
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<h3><a>Learn</a></h3>
<p>We found that high concentration of trp can inhibit expression of sfGFP in negative selection system. However, it can only provide about three times the inhibition effect which cannot achevie screen effect. Possibly, trp repressor has a low expression or trp repressor with trp cannot strongly bind to trp operator at DNA. Same experiments of positive selection system haven't be carry out. </p><br>
<p>After a literature survey, we found that expressing GFP with an enhanced Trp+ promoter may be a good solution.</p><br>
<p>Also, we have successfully make emulsion droplets of high quality. However, results of mock selection is not obvious which means selection system cannot distinguish wild type trp repressor with inactive trp repressor that consistent with results of GFP test.