<p>Indican median is dribbled onto cotton cloth to moisten, and then dried by adding 500 uL of BGL lysate to allow spontaneous oxidation.</p><br>
<br><h3><a>Dye with indigo</a></h3>
<br><h3><a>Dye with tyrian purple</a></h3>
<p>Dyeing solutions (DI water 50 mL; tetrahydrofuran 7.5 mL; NaOH 0.5 g; Na2S2O4 0.5 g and chemical 6BrIG 0.15 g or the produced 6BrIG cell dye) are prepared by heating with stirring on the 75-80 °C hot plate for 15 min. After heating, 0.5 g NH4Cl is added to the dyeing solution and subsequent heating is repeated at 75-80 °C. The fabrics (multi-fabric strips or wool fabrics) are soaked into the solution and dye at 60 °C for 15 min.