Skip to content
Snippets Groups Projects
Commit cc95f5cf authored by Arya Ajit Gohad's avatar Arya Ajit Gohad
Browse files

Update wiki/pages/engineering-design2.html

parent 2847c9ee
No related branches found
No related tags found
No related merge requests found
Pipeline #186451 passed
......@@ -188,21 +188,27 @@ As mentioned we finished the testing aspect of our second engineering cycle just
</p>
<br><br>
<p><h3>F/OD of CspF Mutants - Test Results</h3><br>
<center> <img class="norm" src="https://static.igem.wiki/teams/4303/wiki/design2.jpeg" alt="Image"></center>
<br>
<p><h3>Characterization of CspF Mutants in DH5alpha Strain:</h3</p>
<center><iframe src="https://static.igem.wiki/teams/4303/wiki/nupack-cspf-mutants.pdf" width="100%" height="500px"></iframe></center> </p>
<p><br><center><img class="norm" src="https://static.igem.wiki/teams/4303/wiki/dh5alpha-sdm-graph-cp.jpg" alt="Image"></center></p>
<br>
<br>
<p><h3>Characterization of CspF Mutants in BL21(DE3) Strain:</h3</p>
<center><iframe src="https://static.igem.wiki/teams/4303/wiki/nupack-cspf-mutants.pdf" width="100%" height="500px"></iframe></center>
<br>
</p>
<p><br>
<br><center><img class="norm" src="https://static.igem.wiki/teams/4303/wiki/bar-graph.jpeg" alt="Image"></center></p>
<p>
<br><center><img class="norm" src="https://static.igem.wiki/teams/4303/wiki/bl21-graph-sdm-cp.jpg" alt="Image"></center></p>
<br>
<p><h3>NUPACK CspF Mutants</h3</p>
<center><iframe src="https://static.igem.wiki/teams/4303/wiki/nupack-cspf-mutants.pdf" width="100%" height="500px"></iframe></center>
<br>
<p><h3>NUPACK ANALYSIS</h3></p>
<p><ul><li>From this, the least performing UTR Mutant 23 clearly has a very different structure. Mutant 10 has a similar structure but an RP of 0.21 times higher than Mutant 23. This could be probably because of the GC content of it being different?</li><li>
Mutant 27 has the best expression and Relative Performance of 1.3. It’s structure is very different from the other UTRs. We need to perform 3D corrations.</li><li>
Mutant 26 and 9 have similar structures and similar Relative Performances.</li><li>
Mutant 3 and Mutant 5 have a similar structure and a similar Relative Performance.</li></ul>
<p><ul><li>From this, the least performing UTR SDM23 clearly has a very different structure. SDM10 has a similar structure but an Relative Performance 0.21 times higher than SDM23. This could be probably because of the GC content of it being different?</li><li>
SDM27 has the best expression and Relative Performance of 1.3. It’s structure is very different from the other UTRs. We need to perform 3D corrations.</li><li>
SDM26 and 9 have similar structures and similar Relative Performances.</li><li>
SDM3 and SDM5 have a similar structure and a similar Relative Performance.</li></ul>
</p>
</div>
......
0% Loading or .
You are about to add 0 people to the discussion. Proceed with caution.
Finish editing this message first!
Please register or to comment