diff --git a/wiki/pages/engineering-design2.html b/wiki/pages/engineering-design2.html index 26fb7e8332ebc1ac38d5ebd3d4a527d55ed42927..37b3a863e02764c7a77b708fdc577d09a6e45b55 100644 --- a/wiki/pages/engineering-design2.html +++ b/wiki/pages/engineering-design2.html @@ -188,21 +188,27 @@ As mentioned we finished the testing aspect of our second engineering cycle just </p> <br><br> - <p><h3>F/OD of CspF Mutants - Test Results</h3><br> - <center> <img class="norm" src="https://static.igem.wiki/teams/4303/wiki/design2.jpeg" alt="Image"></center> - + <br> + <p><h3>Characterization of CspF Mutants in DH5alpha Strain:</h3</p> + <center><iframe src="https://static.igem.wiki/teams/4303/wiki/nupack-cspf-mutants.pdf" width="100%" height="500px"></iframe></center> </p> + <p><br><center><img class="norm" src="https://static.igem.wiki/teams/4303/wiki/dh5alpha-sdm-graph-cp.jpg" alt="Image"></center></p> + <br> + <br> + <p><h3>Characterization of CspF Mutants in BL21(DE3) Strain:</h3</p> + <center><iframe src="https://static.igem.wiki/teams/4303/wiki/nupack-cspf-mutants.pdf" width="100%" height="500px"></iframe></center> + <br> </p> - <p><br> - <br><center><img class="norm" src="https://static.igem.wiki/teams/4303/wiki/bar-graph.jpeg" alt="Image"></center></p> + <p> + <br><center><img class="norm" src="https://static.igem.wiki/teams/4303/wiki/bl21-graph-sdm-cp.jpg" alt="Image"></center></p> <br> <p><h3>NUPACK CspF Mutants</h3</p> <center><iframe src="https://static.igem.wiki/teams/4303/wiki/nupack-cspf-mutants.pdf" width="100%" height="500px"></iframe></center> <br> <p><h3>NUPACK ANALYSIS</h3></p> - <p><ul><li>From this, the least performing UTR Mutant 23 clearly has a very different structure. Mutant 10 has a similar structure but an RP of 0.21 times higher than Mutant 23. This could be probably because of the GC content of it being different?</li><li> -Mutant 27 has the best expression and Relative Performance of 1.3. It’s structure is very different from the other UTRs. We need to perform 3D corrations.</li><li> -Mutant 26 and 9 have similar structures and similar Relative Performances.</li><li> -Mutant 3 and Mutant 5 have a similar structure and a similar Relative Performance.</li></ul> + <p><ul><li>From this, the least performing UTR SDM23 clearly has a very different structure. SDM10 has a similar structure but an Relative Performance 0.21 times higher than SDM23. This could be probably because of the GC content of it being different?</li><li> +SDM27 has the best expression and Relative Performance of 1.3. It’s structure is very different from the other UTRs. We need to perform 3D corrations.</li><li> +SDM26 and 9 have similar structures and similar Relative Performances.</li><li> +SDM3 and SDM5 have a similar structure and a similar Relative Performance.</li></ul> </p> </div>