<p>We introduced muscone gas molecule receptors derived from mouse olfactory epithelial cells into chassis bioengineered bacteria. The muscone gas molecule receptor is a G protein coupled receptor in eukaryotic cells, and we chose <i>Saccharomyces cerevisiae</i> as the chassis bioengineering bacterium. By modifying the mating pathway of <i>Saccharomyces cerevisiae</i>, the muscone gas molecule receptor is integrated into the signaling pathway of <i>Saccharomyces cerevisiae</i>. And downstream of the modified mating pathway, lactate dehydrogenase was introduced to alter the anaerobic metabolism pathway of <i>Saccharomyces cerevisiae</i>, synthesizing lactate for the treatment of IBD disease.</p>
<p>Our experimental design consists of two parts: the verification of the mating pathway in <i>Saccharomyces cerevisiae</i> containing muscone gas molecule switches and the modification of the <i>Saccharomyces cerevisiae<i> genome. We independently verified each component of the mating pathway in the modified <i>Saccharomyces cerevisiae</i> and ultimately integrated them. Additionally, we knocked out the original receptor of the <i>Saccharomyces cerevisiae</i> mating signal to eliminate signal interference caused by the yeast’s own growth.</p>
<p>Our experimental design consists of two parts: the verification of the mating pathway in <i>Saccharomyces cerevisiae</i> containing muscone gas molecule switches and the modification of the <i>Saccharomyces cerevisiae</i> genome. We independently verified each component of the mating pathway in the modified <i>Saccharomyces cerevisiae</i> and ultimately integrated them. Additionally, we knocked out the original receptor of the <i>Saccharomyces cerevisiae</i> mating signal to eliminate signal interference caused by the yeast’s own growth.</p>
