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     <p>In the galactose-induced experimental group, the fluorescence intensity and proportion of the GFP reporter gene under muscone induction were significantly higher than those in the control group without muscone. In the glucose control group, there was no significant difference in the fluorescence intensity and proportion of the GFP reporter gene between the muscone-induced experimental group and the control group. The experiment preliminarily proves the effectiveness of the introduced muscone molecular switch in <i>Saccharomyces cerevisiae</i>.</p>
     <p>At the same time, we found that compared to the galactose-induced experimental group, the glucose control group showed a higher background noise of mating signals in <i>Saccharomyces cerevisiae</i>. In our subsequent experimental design, we knocked out the original receptor of the <i>Saccharomyces cerevisiae</i> mating signal pathway, which reduced the background signal intensity of the <i>Saccharomyces cerevisiae</i> mating signal pathway and improved the reliability of the system.</p>
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       <p style="text-align: center; font-size: 0.9em; margin-top: 10px;">fig 8 Muscone molecular switch fluorescence signal test, A. Galactose-induced, add muscone organic solution. B. Galactose-induced, without muscone. C. Glucose control group, add muscone organic solution. D. Glucose control group, without muscone.</p>
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