<p>Biosafety is an important part of project design. In the case of Muscure, a clinical application project, we primarily focus on project safety design from four aspects: biological design, production, transportation, and use.</p>
<p>Biosafety is an important part of project design. In the case of <b>Muscure</b>, a clinical application project, we primarily focus on project safety design from four aspects: biological design, production, transportation, and use.</p>
<p>We have also consulted relevant professionals regarding project safety. To learn more, enter Human Practices. </p>
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<h3>Design</h3>
<p>In project design, we double our biosecurity through nutritional deficiencies and suicide genes. We used four deficiencies of yeast with four nutritional deficiencies as the engineered bacteria, and two different nutritional deficiencies remained after the transformation of plasmids. As a result, engineered yeasts can only survive under laboratory conditions at present, effectively preventing the leakage of engineered bacteria into the environment. For more information on safety design related to nutrient deficiency, enter <ahref="https://2024.igem.wiki/Tsinghua/description"style="color: #FF5151">Description </a>.</p>
<p>In project design, we double our biosecurity through nutritional deficiencies and suicide genes. We used four deficiencies of yeast with four nutritional deficiencies as the engineered bacteria. The yeast chassis we use has defects in lysine, leucine, uracil and histidine synthesis. After transforming plasmids, our engineered yeast contains defects in histidine and uracil synthesis. As a result, engineered yeasts can only survive under laboratory conditions at present, effectively preventing the leakage of engineered bacteria into the environment. </p>
<p>Before participating in wet experiments, all team members have signed safety protocol agreements to demonstrate their understanding of laboratory safety protocols and their commitment to responsible behavior.</p>
<p>To constantly remind everyone of their safety during the experiment, safety signs are posted throughout the laboratory. Specific instructions for the use of the equipment are indicated on the relevant experimental instruments, such as a waste tank for discarding special liquids.</p>
<p>We have implemented a "last person" system, in which the last person to leave the laboratory is responsible for checking whether all experimental instruments are organized or closed and must sign a confirmation form. This ensures our commitment to environmental protection and safety on a daily basis.</p>
<p>Regular team meetings also involve discussions and summaries of encountered experimental safety and protocol-related issues, such as proper procedures for waste segregation. This continuous practice deepens our understanding of laboratory safety regulations.</p>