<p>We expressed the gRNA targeting the STE2 receptor gene and the Cas9 protein through a constitutive promoter and screened the successfully transformed yeast using leu nutritional deficiency. The genome of the successfully transformed <i>Saccharomyces cerevisiae</i> strains was sequenced to screen for strains with a successful knockout of STE2, followed by the transformation of the muscone molecular switch signaling pathway.</p>
<p>We expressed the gRNA targeting the STE2 receptor gene and the Cas9 protein through a constitutive promoter and screened the successfully transformed yeast using leu nutritional deficiency. The genome of the successfully transformed <i>Saccharomyces cerevisiae</i> strains was sequenced to screen for strains with a successful knockout of STE2, followed by the transformation of the muscone molecular switch signaling pathway.</p>
<p>Aim:</p>
<p><b>Aim:</b></p>
<p>To remove the interference of <i>Saccharomyces cerevisiae</i>'s own growth and mating signals on the secretory system.</p>
<p>To remove the interference of <i>Saccharomyces cerevisiae</i>'s own growth and mating signals on the secretory system.</p>
