<p>Through our efforts, we have obtained some experimental results to support our system design. We divided the colonization system into the TtrRS system and the Als3 protein and tested their functionality through a series of wet lab experiments. Finally, we plan to integrate them to test the effectiveness of the complete colonization system. You can see our experimental results in the following content.</p>
<h3>Tetrathionate sensor TtrSR</h3>
<p>After successfully transforming the pYES2-SV40-ttrS and pESC-SV40-ttrR-PttrB-EGFP plasmids into Saccharomyces cerevisiae, we selected three colonies with good conditions for amplification. Then, the same clone was divided into two groups, one group was induced with 1mM tetrathionate, and the other group was used as a control without inducer. After 12 hours of induction, an appropriate amount of bacterial solution was prepared into temporary slides and the fluorescence intensity was tested using a fluorescence confocal microscope. After obtaining the fluorescence confocal microscopy images, we calculated the ratio of total fluorescence intensity to the number of cells. The images were processed using Image J, and statistical graphs were plotted using GraphPad Prism.</p>
<p>After successfully transforming the pYES2-SV40-ttrS and pESC-SV40-ttrR-PttrB-EGFP plasmids into Saccharomyces cerevisiae, we selected three colonies with good conditions for amplification. Then, the same clone was divided into two groups, one group was induced with 2mM tetrathionate, and the other group was used as a control without inducer. After 24 hours of induction, an appropriate amount of bacterial solution was prepared into temporary slides and the fluorescence intensity was tested using a fluorescence confocal microscope. After obtaining the fluorescence confocal microscopy images, we calculated the ratio of total fluorescence intensity to the number of cells. The images were processed using Image J, and statistical graphs were plotted using GraphPad Prism.</p>
