<p>The lactate secretion experiment has confirmed the feasibility of our treatment project. Next, we need to find a molecular switch that matches it. As a basic structure in our design, the molecular switch plays a crucial role. It is through the switch that we can control the timing and quantity of administration to patients. More importantly, to address the current medication challenges faced by IBD patients, the upstream drug delivery switch we designed needs to avoid oral administration whenever possible and should be easy to operate. This will provide patients with a better medication experience. After literature investigation, we finally decided to use the muscone receptor as a candidate for the molecular switch which response of diffusible gas muscone. We then need to verify that the muscone receptor can function effectively in yeast cells.</p>
<h4>Cycle1</h4>
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<h5>Test</h5>
<p>We induced the double-transformed yeast with galactose to express the muscone receptor and the corresponding Gα protein. Then, we induced muscone and observed the expression of GFP fluorescence signal in yeast cells under a confocal microscope. The results are as follows. We can find that in the group expressing the muscone receptor, adding muscone can significantly increase the fluorescence expression of yeast cells. However, what is frustrating is that in the group induced by glucose, no matter whether muscone is added or not, yeast has a very high fluorescence level.</p>
