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       <h3>Tetrathionate sensor TtrSR</h3>
       <p>After successfully transforming the pYES2-SV40-ttrS and pESC-SV40-ttrR-PttrB-EGFP plasmids into Saccharomyces cerevisiae, we selected three colonies with good conditions for amplification. Then, the same clone was divided into two groups, one group was induced with 1mM tetrathionate, and the other group was used as a control without inducer. After 12 hours of induction, an appropriate amount of bacterial solution was prepared into temporary slides and the fluorescence intensity was tested using a fluorescence confocal microscope. After obtaining the fluorescence confocal microscopy images, we calculated the ratio of total fluorescence intensity to the number of cells. The images were processed using Image J, and statistical graphs were plotted using GraphPad Prism.</p>
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         <p style="text-align: center; font-size: 0.9em; margin-top: 10px;">fig 10 Fluorescence confocal microscopy imaging results (s: control group without inducer; s+: K<sub>2</sub>O<sub>6</sub>S<sub>4</sub> added)</p>
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