<p>Due to time and other constraints, we may not be able to achieve a more comprehensive design and analysis of MusCure. However, we hope to make further efforts in the following aspects to make this project more complete:</p>
<h3>Muscone solecular switch </h3>
<h3>Muscone molecular switch </h3>
<p>Traditional fermentation engineering uses Pichia pastoris for fermentation. When methanol is used as a carbon source, the expression of aldehyde oxidase (AOX) is greatly increased, and traditional fermentation engineering takes advantage of this characteristic to make the yeast express target genes located near the AOX gene. However, methanol has certain biological toxicity. Muscone itself is non-toxic and harmless, and as a water-soluble molecule, its recovery and reuse are relatively simple. We are exploring a system that uses muscone as a molecular switch, hoping to apply it to yeast fermentation engineering after further research.</p>
<h3>Drug selection</h3>
<p>In addition to lactate, we also plan to try some other small molecule drugs as target proteins for our engineered bacteria, further exploring the potential of MusCure in treating IBD and other diseases.</p>
