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     <h3>Muscone molecular switch</h3>
-    <p>We selected muscone gas molecules as the upstream control signal for our therapy system. We used the muscone receptor sequence from mouse olfactory epithelial cells, as employed by the Ye Haifeng team; for details, please refer to the <a href="https://2024.igem.wiki/tsinghua/description" target="_blank"  style="color: #FF5151 ;">description</a>, and introduced it into the plasmid system expressed in Saccharomyces cerevisiae. We chose the mating pathway in Saccharomyces cerevisiae as the transmission pathway for the muscone signal within Saccharomyces cerevisiae. Based on the Benjamin M Scott team’s optimization, we replaced the C-terminal five amino acids of the Gα protein in the original mating pathway, allowing the muscone receptor to be integrated into the Saccharomyces cerevisiae mating pathway.</p>
+    <p>We selected muscone gas molecules as the upstream control signal for our therapy system. We used the muscone receptor sequence from mouse olfactory epithelial cells, as employed by the Ye Haifeng team <sup>[1]</sup>; for details, please refer to the <a href="https://2024.igem.wiki/tsinghua/description" target="_blank"  style="color: #FF5151 ;">description</a>, and introduced it into the plasmid system expressed in Saccharomyces cerevisiae. We chose the mating pathway in Saccharomyces cerevisiae as the transmission pathway for the muscone signal within Saccharomyces cerevisiae. Based on the Benjamin M Scott team's optimization<sup>[2]</sup>, we replaced the C-terminal five amino acids of the Gα protein in the original mating pathway, allowing the muscone receptor to be integrated into the Saccharomyces cerevisiae mating pathway.</p>
     <p>We used the galactose promoter to induce the expression of the muscone signal receptor and the optimized Gα protein, and screened the successfully transformed Saccharomyces cerevisiae with a His nutritional deficiency. By controlling the induction conditions of galactose and muscone, we tested the effectiveness of the muscone gas molecule switch. For details, please refer to the protocol.</p>
     <p>Aim:</p>
     <p>To validate the effectiveness of the muscone gas molecule switch in Saccharomyces cerevisiae.</p>
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     <h3>Mating pathway pFUS1 promoter</h3>
-    <p>We chose the mating pathway in Saccharomyces cerevisiae as the conduit for muscone signaling in yeast. Using the mating pathway’s pFUS1 promoter, we expressed the downstream lactate dehydrogenase to alter the anaerobic metabolic pathway of Saccharomyces cerevisiae, secreting lactic acid for the treatment of IBD. Initially, we designed a plasmid with the pFUS1 promoter expressing the GFP reporter gene and screened the successfully transformed yeast using Ura nutritional deficiency. We then tested the effectiveness of the muscone molecular switch using confocal microscopy; for details, please refer to the protocol. Subsequently, we designed the pFUS1 promoter to express lactate dehydrogenase from E. coli. By co-transforming it with Muscone Receptor & Gα (pESC) into Saccharomyces cerevisiae, we achieved the construction of the complete pathway.</p>
+    <p>We chose the mating pathway in Saccharomyces cerevisiae as the conduit for muscone signaling in yeast. Using the mating pathway’s pFUS1 promoter, we expressed the downstream lactate dehydrogenase to alter the anaerobic metabolic pathway of Saccharomyces cerevisiae, secreting lactic acid for the treatment of IBD<sup>[3]</sup>. Initially, we designed a plasmid with the pFUS1 promoter expressing the GFP reporter gene and screened the successfully transformed yeast using Ura nutritional deficiency. We then tested the effectiveness of the muscone molecular switch using confocal microscopy; for details, please refer to the protocol. Subsequently, we designed the pFUS1 promoter to express lactate dehydrogenase from E. coli. By co-transforming it with Muscone Receptor & Gα (pESC) into Saccharomyces cerevisiae, we achieved the construction of the complete pathway.</p>
     <p>Aim:</p>
     <p>To check the reporter signals downstream of the muscone molecular switch.</p>
     <p>To check the synthesis of the secretion system downstream of the muscone molecular switch.</p>
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     <h2 id="Reference">
       <h2>Reference</h2>
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+      <p>[1] Wu X, Yu Y, Wang M, Dai D, Yin J, Liu W, Kong D, Tang S, Meng M, Gao T, Zhang Y, Zhou Y, Guan N, Zhao S, Ye H. AAV-delivered muscone-induced transgene system for treating chronic diseases in mice via inhalation. Nat Commun. 2024 Feb 6;15(1):1122. doi: 10.1038/s41467-024-45383-z. PMID: 38321056; PMCID: PMC10847102.</p>
+      <p>[2] Scott BM, Gutiérrez-Vázquez C, Sanmarco LM, da Silva Pereira JA, Li Z, Plasencia A, Hewson P, Cox LM, O'Brien M, Chen SK, Moraes-Vieira PM, Chang BSW, Peisajovich SG, Quintana FJ. Self-tunable engineered yeast probiotics for the treatment of inflammatory bowel disease. Nat Med. 2021 Jul;27(7):1212-1222. doi: 10.1038/s41591-021-01390-x. Epub 2021 Jun 28. PMID: 34183837.</p>
+      <p>[3] Sanmarco LM, Rone JM, Polonio CM, Fernandez Lahore G, Giovannoni F, Ferrara K, Gutierrez-Vazquez C, Li N, Sokolovska A, Plasencia A, Faust Akl C, Nanda P, Heck ES, Li Z, Lee HG, Chao CC, Rejano-Gordillo CM, Fonseca-Castro PH, Illouz T, Linnerbauer M, Kenison JE, Barilla RM, Farrenkopf D, Stevens NA, Piester G, Chung EN, Dailey L, Kuchroo VK, Hava D, Wheeler MA, Clish C, Nowarski R, Balsa E, Lora JM, Quintana FJ. Lactate limits CNS autoimmunity by stabilizing HIF-1α in dendritic cells. Nature. 2023 Aug;620(7975):881-889. doi: 10.1038/s41586-023-06409-6. Epub 2023 Aug 9. PMID: 37558878; PMCID: PMC10725186.</p>
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