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Commit 45361a26 authored by HouTeng Chan's avatar HouTeng Chan
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- /wiki/pages/safety.html
- /wiki/pages/contribution.html
- /wiki/pages/therapy-system.html
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<h2 id="Parts">
<h2>New synthetic biology composite part</h2>
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<h3>Muscone-gated molecular switch in Saccharomyces cerevisiae</h3>
<h3>Muscone-gated molecular switch in <i>Saccharomyces cerevisiae</i></h3>
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<img src="https://static.igem.wiki/teams/5187/wiki-contribution-fig/2.jpg" alt="ibd_figure" class="shadowed-image" style="width: 45%; max-width: 400px;">
<p style="text-align: center; font-size: 0.9em; margin-top: 10px;">fig 1 The design of Muscone-gated molecular switch in Saccharomyces cerevisiae</p>
<p style="text-align: center; font-size: 0.9em; margin-top: 10px;">fig 1 The design of Muscone-gated molecular switch in <i>Saccharomyces cerevisiae</i></p>
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<p>One of our standout contributions to synthetic biology tools is the construction of a Muscone-gated molecular switch in Saccharomyces cerevisiae BBa_K5187006. The muscone receptor is a type of GPCR derived from mice. We designed the corresponding Gα protein by modifying the amino acid sequence so that it can trigger the mating pathway in Saccharomyces cerevisiae, thereby further inducing the expression of downstream genes.</p>
<p>It is worth noting that this molecular switch in Saccharomyces cerevisiae is an original contribution from our team. Although in our experiment it was applied to secrete lactic acid for the treatment of IBD, this downstream element can be replaced with any other gene to achieve different biological functions. We believe that this minute, efficient, and cost-effective molecular switch will have broad application scenarios in related industries.</p>
<p>One of our standout contributions to synthetic biology tools is the construction of a Muscone-gated molecular switch in <i>Saccharomyces cerevisiae</i> BBa_K5187006. The muscone receptor is a type of GPCR derived from mice. We designed the corresponding Gα protein by modifying the amino acid sequence so that it can trigger the mating pathway in <i>Saccharomyces cerevisiae</i>, thereby further inducing the expression of downstream genes.</p>
<p>It is worth noting that this molecular switch in <i>Saccharomyces cerevisiae</i> is an original contribution from our team. Although in our experiment it was applied to secrete lactic acid for the treatment of IBD, this downstream element can be replaced with any other gene to achieve different biological functions. We believe that this minute, efficient, and cost-effective molecular switch will have broad application scenarios in related industries.</p>
<p>Simultaneously, we have conducted molecular dynamics simulations to predict the binding of muscone molecules to their receptors, providing a more detailed and quantitative explanation of the biological process of the muscone-gated molecular switch. This aims to assist future research teams in obtaining more molecular dynamics information about this molecular switch and developing more functions of the switch. For more information, please refer to <a href="https://2024.igem.wiki/Tsinghua/model#topic2" style="color: #FF5151" >Model: Binding</a>.</p>
<p>For more information about the Muscone-gated molecular switch in Saccharomyces cerevisiae, please refer to <a href="https://2024.igem.wiki/Tsinghua/parts" style="color: #FF5151">Parts</a>.</p>
<p>For more information about the Muscone-gated molecular switch in <i>Saccharomyces cerevisiae</i>, please refer to <a href="https://2024.igem.wiki/Tsinghua/parts" style="color: #FF5151">Parts</a>.</p>
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<p>In order to popularize knowledge about molecular biology experiments used in synthetic biology, we have registered accounts on various video media platforms such as Tiktok, Bilibili, and YouTube. Based on the experimental content of our project, we have produced and released a series of instructional videos on molecular biology experimental skills in both Chinese and English versions, helping more people learn related experimental techniques.</p>
<p>For more information about the instructional videos, please refer to <a href="https://2024.igem.wiki/Tsinghua/education#Public" style="color: #FF5151" >Education: Public</a>.</p>
<h3>About new experimental protocol</h3>
<p>Our project uses Saccharomyces cerevisiae as the chassis organism. During the design and experimental process of our project, we have optimized and improved the original experimental operation procedures for the transformation, culture, and induction of Saccharomyces cerevisiae. This not only helped us achieve better results in the project experiments but can also save experimental time and costs for future iGEM teams and members who wish to use brewing yeast as the chassis organism.</p>
<p>Our project uses <i>Saccharomyces cerevisiae</i> as the chassis organism. During the design and experimental process of our project, we have optimized and improved the original experimental operation procedures for the transformation, culture, and induction of <i>Saccharomyces cerevisiae</i>. This not only helped us achieve better results in the project experiments but can also save experimental time and costs for future iGEM teams and members who wish to use brewing yeast as the chassis organism.</p>
<p>For more information about the new protocol, please refer to <a href="https://2024.igem.wiki/Tsinghua/therapy-system#Protocol" style="color: #FF5151" >Protocol</a>.</p>
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<h3>Project</h3>
<p>According to our design, therapeutic engineered yeast will be produced in a large-scale specialized microbiological facility. Specialized facilities must have the appropriate equipment and trained personnel. What’s more, the laboratory and premises during the production process must adhere to international biosafety standards and ensure strict biosafety levels, such as BSL-2 or BSL-3. The cultivation of Saccharomyces cerevisiae must take place in controlled bioreactors to minimize the risk of leakage. Personnel must undergo specialized training and strictly follow operating procedures, including wearing protective gear and using biosafety cabinets. Waste must be handled and disposed of in accordance with the standards for biohazardous waste to prevent any leakage of biological materials.</p>
<p>According to our design, therapeutic engineered yeast will be produced in a large-scale specialized microbiological facility. Specialized facilities must have the appropriate equipment and trained personnel. What’s more, the laboratory and premises during the production process must adhere to international biosafety standards and ensure strict biosafety levels, such as BSL-2 or BSL-3. The cultivation of <i>Saccharomyces cerevisiae</i> must take place in controlled bioreactors to minimize the risk of leakage. Personnel must undergo specialized training and strictly follow operating procedures, including wearing protective gear and using biosafety cabinets. Waste must be handled and disposed of in accordance with the standards for biohazardous waste to prevent any leakage of biological materials.</p>
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