<p><i>Saccharomyces cerevisiae</i> is a premier chassis organism for our project. As a kind of common yeast found in human gut, <i>Saccharomyces cerevisiae</i> is considered safe for human and has been extensively studied for its potential application in synthetic biology. As a eukaryotic organism, it possesses more complex and precise mechanisms for gene expression regulation, and various modification approaches also enhance its ability to secrete target proteins. Of note, it has been employed to prevent and treat various diarrheal disorders due to its capability to reshape the balance of gut flora. We believe that <i>Saccharomyces cerevisiae</i> is the best chassis organism to achieve our goal.</p>