Merge branch 'main' of ssh://gitlab.igem.org/2024/bielefeld-cebitec

src/contents/results.tsx

@@ -53,12 +53,31 @@ export function Results() {
           <p>The transfection efficiency was much better. Our proof-of-concept was working correctly. The reporter system pDAS12189 only led to production of a fluorescent signal when co transfected with a prime editing complex as pCMV-PE2.</p> 
       </Subesction>
       <Subesction title="Mechanism" id="Results2">
-          <H4 text="Goals"/>
-          <p>text</p> 
-          <H4 text="Workflow"/>
-          <p>text</p> 
-          <H4 text="Conclusion"/>
-          <p>text</p> 
+          <H4 text="pegRNA 1"/>
+          <H5 text="Workflow"/>
+          <p>With this experiment we wanted to compare the efficiency of pegRNAs with and without silent edits.</p> 
+          <H5 text="Conclusion"/>
+          <p>The FACS analysis shows that pegRNA without silent edits (pegRNA1) had a 2.05 times higher transfection efficiency than pegRNA with silent edits (pegRNA2).</p> 
+          <H4 text="pegRNA 2"/>
+          <H5 text="Workflow"/>
+          <p>Cotransfection of pPEAR_CFTR and PE2 and also 1 of the 14 pegRNAs to compare the transfection efficiency of all of our designed pegRNAs.</p> 
+          <H5 text="Conclusion"/>
+          <p>The pegRNAs lead to differing amounts of cells showing fluorescence, which, assuming comparable transfection efficiencies, indicates varying prime editing efficiency. The pegRNA7 showed the highest transfection efficiency (see Figure X).</p>
+          <H4 text="pegRNA 3"/>
+          <H5 text="Workflow"/>
+          <p>We tried to transfect CFBE41o- cells with pDAS12124-preedited, our internal positive control, to check if a transfection of this cell line is possible. Furthermore we tried to co transfect the CFBE41o- with pPEAR_CFTR, PE6c and pegRNA4.</p> 
+          <H5 text="Conclusion"/>
+          <p>Transfection of CFBE41o- with pDAS12124-preedited was successful (see Figure X). After 24 hours a successful co transfection of pPEAR_CFTR with PE6c and pegRNA4 was visible, although the transfection efficiency was really bad (see Figure X).</p>
+          <H4 text="pegRNA 4"/>
+          <H5 text="Workflow"/>
+          <p>pCMV-PE2 was co transfected with pDAS12489 and pCMV-PE_CO-Mini was co transfected with pDAS12489 in HEK293 cell line.</p> 
+          <H5 text="Conclusion"/>
+          <p>The FACS results show that transfection with pCMV-PE2 as the prime editing complex had editing efficiency of 52.90% when normalized on pDAS12124-preedited. When pCMV-PE_CO-Mini was used as a prime editing complex it had a transfection efficiency of 2.54% (see Figure X).</p>
+          <H4 text="pegRNA 5"/>
+          <H5 text="Workflow"/>
+          <p>We compared the 3 different Prime Editing complexes (pCMV-PE2, pCMV-PE2_CO-Mini & pCMV-PE6c) to check which one has the best transfection efficiency.</p> 
+          <H5 text="Conclusion"/>
+          <p>The FACS measurement shows the fluorescence rate cells co-transfected with pDAS12489 and pCMV-PE6c as a prime editing complex. The editing efficiency off PE6c was by far the highest (81.88%) (see Figure X). The efficiency was 1.55 higher than the efficiency when pCMV-PE2 was used as prime editing complex (see Figure X).</p> 
       </Subesction >
       <Subesction  title="Delivery" id="Results3">
       <H4 text="RNA Synthesis"/>