Merge branch 'main' of ssh://gitlab.igem.org/2024/bielefeld-cebitec

src/App/mediarules.css

@@ -147,6 +147,10 @@
     position:initial!important;
     font-size: 0.5em !important;
 
+  }
+  img[src*="200k-anim-transparent-bg.gif"] {
+    width: 100%;
+    height: auto;
   }
     /***Biosafty***/
     #safehead {

src/contents/Human Practices/Conclisuin.tsx

@@ -242,7 +242,20 @@ function AnalyseBharti(){
      <Collapsible title="Dr. Nikhil Bharti – Expert in Primary culture " id="bhartianalyseC">
          <p>Dr. Nikhil Bharti's expertise helped us overcome a major challenge with our CF cell cultures, which were consistently becoming contaminated with fungi due to their instability. He recommended a specific antibiotic composition effective against fungal infections. </p>
         <p>We immediately applied this antibiotic mixture to our cell cultures, which resolved the contamination issue and allowed us to maintain stable CF cell lines. This breakthrough enabled us to proceed with testing our prime editing approach effectively. Thanks to Dr. Bharti's insights, we advanced our project to a stage where we reached the limit of in vitro testing, and the next step would be potential animal studies. </p>
-        
+        <div className="row align-items-center">
+                <div className="col">
+                    <figure>
+                    <img src="https://static.igem.wiki/teams/5247/photos/hp/cell-culture-mucos.webp" alt="" />
+                    <figcaption>Figure 1. Primary cell culture without antibiotic treatment.</figcaption>
+                    </figure>
+                </div> 
+                <div className="col">
+                    <figure>
+                    <img src="https://static.igem.wiki/teams/5247/photos/cell-culture-after-antibiotic-treatment.webp" alt=""/>
+                    <figcaption>Figure 2. Primary cell culture with antibiotic treatment.</figcaption>
+                    </figure>
+                </div>
+            </div>
         <p><strong>Contamination challenge:</strong> Helped us address a major issue with fungal contamination in our CF cell cultures, which were unstable and prone to infection.</p>
 
 <p><strong>Antibiotic solution:</strong> Recommended a specific antibiotic composition effective against fungal infections.</p>
@@ -275,6 +288,16 @@ function AnalyseIgnatova(){
                     </figure>
                 </div>
             </div>
+<p><strong>Introduction through iGEM Hamburg:</strong> Collaborated with Prof. Ignatova, a leading expert in CF research, to deepen our understanding of the disease.</p>
+
+<p><strong>Alternative cell models:</strong> After HEK cells from Leuven proved unsuitable, we consulted Prof. Ignatova, who provided access to the CFBE41o- cell line, immortalized CF cells from a patient.</p>
+
+<p><strong>Cell acquisition and cultivation:</strong> Obtained the CFBE41o- cells with permission from Prof. Karl Kunzelmann, successfully cultivated them in our lab, though they required extended acclimation.</p>
+
+<p><strong>Future testing plans:</strong> Plan to use these cells for patch-clamp experiments to validate our prime editing approach.</p>
+
+<p><strong>Broadened perspective on gene therapy:</strong> Discussions with Prof. Ignatova introduced us to alternative therapeutic strategies, such as recoding tRNAs, expanding our understanding of potential treatments for CF.</p>
+
 
      </Collapsible>
     ) 
@@ -341,12 +364,23 @@ function AnalyseMattijs(){
                     </figure>
                 </div>
             </div>
+<p><strong>Consultation because of test system:</strong> HEK cells with corresponding CFTR mutation derived from Leuven were successfully cultured in our lab for testing purposes.</p>
+
+<p><strong>Understanding test cell lines:</strong> Explained the system behind the test cell lines, enabling us to adapt pegRNA engineering for our Prime Editing complex.</p>
+
+<p><strong>Integration of TevoPreQ1:</strong> Helped us incorporate the structural motif TevoPreQ1 into the pegRNA, significantly enhancing the efficiency of the Prime Editor.</p>
+
+<p><strong>Critical improvement:</strong> This enhancement was successfully tested and demonstrated in our results, marking a major advancement in our project.</p>
+
+<p><strong>Switching testing systems: </strong>After patch clamp measurements revealed that HEK cells were unsuitable for further experiments, we adjusted our system and switched to Ignatova cells from Hamburg for continued testing.</p>
+
+    
      </Collapsible>
     ) 
  }
 function AnalyseMichaela(){
     return(
-     <Collapsible title="Michaela Bienert – Scientific Sales Representative at Stemcell Technologies" id="michaelaanalyseC">
+     <Collapsible title="Dr. Michaela Bienert – Scientific Sales Representative at Stemcell Technologies" id="michaelaanalyseC">
         <p>Through hands-on experience with Michaela Bienert and Julie Watson, we gained valuable insights into the cultivation methods of Air-Liquid Interface (ALI) and apical-out organoids. We evaluated the advantages and limitations of different culturing techniques, which enabled us to make informed decisions about their implementation in our research. With the protocols and resources provided, we successfully generated ALI cultures and organoids from primary cells of CF patients and healthy control donors, which we were able to test throughout the course of our project. </p>
         <div className="row align-items-center">
                 <div className="col">
@@ -356,8 +390,7 @@ function AnalyseMichaela(){
                 </div> 
                 <div className="col gif-wrapper">
                     <figure>
-                    <img src="https://video.igem.org/w/5Ux9MfiKnLYjEwSDW3VzUQ" alt=""/>
-                    
+                    <iframe title="Apical Out Organoids vs. Air Liquid Culture" width="560" height="315" src="https://video.igem.org/videos/embed/27ba6dab-ac52-4259-9a0e-153c9e626804?loop=1&amp;autoplay=1&amp;muted=1&amp;title=0" frameBorder="0" allowFullScreen={true} sandbox="allow-same-origin allow-scripts allow-popups allow-forms"></iframe>
                     </figure>
                 </div>
             </div>
@@ -460,14 +493,20 @@ function AnalyseMoor(){
         <p>We gained valuable insights into the unique properties of chitosan, a cationic polymer with significant potential to stabilize RNA in our lipid nanoparticle (LNP) formulations. Chitosan offers robust protection against RNases and exhibits heat stability, making it suitable for processing methods like spray drying. Additionally, its mucoadhesive properties enable optimal choice as LNP component. </p>
         <p>A critical insight was the necessity for chitosan to be in an acidic environment (pH 4-6) to maintain its positive charge, which is essential for effective RNA interaction. While it cannot replace PEG due to its hydrophilic nature, chitosan is ideal for forming RNA-chitosan complexes, which can then be encapsulated within LNPs. This approach significantly enhances RNA stability during spray drying, a method we intend to further test in collaboration with <a href="https://rnhale.com/">Rnhale</a>. </p>
         <p> In terms of implementation, Benjamin educated us on the chemical and structural properties of chitosan, reinforcing our approach to improve stability, particularly against heat, in our LNP formulations. He provided guidance on formulating chitosan-RNA complexes and developed a protocol for integrating them into our LNP formulation without affecting the charge of the nanoparticles. Additionally, he supplied us with chitosan in various sizes, enabling us to test different chitosan complexes for optimal results. </p>
-            <div className="row">
-            <div className="col">
-            <figure>
-            <img src="https://static.igem.wiki/teams/5247/integrated-human-practices/chitosan.webp" style={{height:"50%", width:"auto"}} alt=""/>
-            
-            </figure>
-            </div>
+        <div className="row align-items-center">
+                <div className="col">
+                    <figure>
+                    <img src="https://static.igem.wiki/teams/5247/integrated-human-practices/chitosan.webp" alt="" />
+                    </figure>
+                </div> 
+                <div className="col">
+                    <figure>
+                    <img src="//TODO (ISI)" alt=""/>
+                    
+                    </figure>
+                </div>
             </div>
+        
         <p><strong>Chitosan properties:</strong> Provided valuable insights into chitosan, a cationic polymer with strong potential to stabilize RNA in lipid nanoparticle (LNP) formulations due to its robust protection against RNases and heat stability.</p>
 
 <p><strong>Mucoadhesive properties:</strong> Highlighted chitosan's mucoadhesive characteristics, making it an ideal component for LNPs in terms of RNA delivery.</p>

src/contents/Human Practices/Overwiev.tsx

@@ -9,10 +9,10 @@ export function HPOverview(){
                 <h2 id="Overview"></h2>
                 
                 <img src="https://static.igem.wiki/teams/5247/photos/overview-ihp-final-final-min.webp" alt="Stakeholder Overview"></img>
-                <figcaption><strong>Figure: Visualization of the Human-Centric Approach by iGEM Bielefeld-CeBiTec 2024</strong><p>Our project has been supported by over 80 individuals and institutions, enhancing our integrative human practices. We are deeply grateful for the invaluable feedback and the collective effort of everyone who has contributed to our vision of safe and precise gene therapy through PreCyse. Thank you for your support!
+                <strong>Figure: Visualization of the Human-Centric Approach by iGEM Bielefeld-CeBiTec 2024</strong><p>Our project has been supported by over 80 individuals and institutions, enhancing our integrative human practices. We are deeply grateful for the invaluable feedback and the collective effort of everyone who has contributed to our vision of safe and precise gene therapy through PreCyse. Thank you for your support!
                 
                 </p>
-                </figcaption>
+                
 
                 <span id="hp-quote"><BlockQuoteB
                 text="Human Practices is the study of how your work affects the world, and how the world affects your work."