Merge branch 'main' of ssh://gitlab.igem.org/2024/bielefeld-cebitec

src/contents/engineering.tsx

@@ -8,6 +8,7 @@ import { Collapsible } from "../components/Collapsible";
 import { useNavigation } from "../utils";
 import { TabScrollLink } from "../components/Link";
 import { InfoBox } from "../components/Boxes";
+import { DownloadLink } from "../components/Buttons";
 
 
 
@@ -519,6 +520,9 @@ export function Engineering() {
                         <H4 text="DMG-PEG2000 vs mPEG-2000-DSPE" id="text" /> 
                         While mPEG-2000-DSPE has traditionally been used for stabilizing LNPs and enhancing delivery efficiency, we decided to incorporate DMG-PEG2000 into our SORT LNP-based AirBuddy due to its superior properties. DMG-PEG2000 offers better biodegradability and enhanced stability in pulmonary applications. Unlike mPEG-2000-DSPE, which tends to accumulate in the body and may lead to immune activation over time, DMG-PEG2000 is known for its faster clearance and reduced potential for long-term toxicity. For lung-specific delivery, where stability and safety are critical, DMG-PEG2000 ensures that the nanoparticles remain stable long enough to deliver the therapeutic material effectively, but also degrade at a rate that minimizes unwanted immune responses. This makes DMG-PEG2000 a more suitable choice for therapies targeting CFTR-related diseases, where precise and safe delivery to the lungs is essential for treatment success. 
                         <p>Details about the biosafety aspects of our LNP can be read // <a onClick={() => goToPageAndScroll ('Biosafety2H', '/safety')}> here </a>.  </p>
+                        <p>Click this Button to gain the LNP Handbook</p>
+                        <DownloadLink url="https://static.igem.wiki/teams/5387/liposomes-handbook.pdf" fileName="liposomes-handbook.pdf" /> 
+                        
                         <H4 text="Conclusion" id="text" /> 
                         We use DMG-PEG2000 in our SORT LNP-based AirBuddy because of its superior biodegradability, enhanced stability, and reduced risk of immune system activation. By building on insights from experts and incorporating principles from Wang’s LNP work, we’ve tailored our nanoparticles for lung-specific delivery. This choice ensures that our formulations remain stable long enough to deliver the therapeutic payload effectively while minimizing potential long-term toxicity. This balance is crucial for pulmonary applications, where DMG-PEG2000 outperforms alternatives like mPEG-2000-DSPE, making it the ideal choice for treating CFTR-related lung diseases. 
                         {/* <H4 text="text" id="text" /> 
@@ -548,7 +552,7 @@ export function Engineering() {
                         <H5 text="LNP Assembly" id="text" />
                             <p>Our LNP can be formulated using various methods depending on the scale of production, including pipette mixing, vortex mixing, or microfluidic mixing. After mixing the lipids with mRNA in carefully controlled ratios, the mixture is typically dialyzed to remove organic solvents like ethanol and citrate buffer. The choice of lipid composition and preparation method influences the tissue-targeting capabilities of the LNPs, allowing for selective delivery to organs like the liver, lungs, or spleen. For more detailed information on formulation methods and lipid selection, refer to our LNP Handbook deigned in <a onClick={() => goToPageAndScroll ('handbook', '/human-practices')}> cooperation with iGEM Team Linköping </a> and others.</p>
                         <H5 text="Spray drying Procedure" id="text" />
-                            <p>By combining these components with the spray drying method from RNhale [2] we offer a versatile and efficient method for delivering mRNA therapeutics to the lung, paving the way for gene therapy, especially our Prime Guide. The effective delivery of the prime editing complex is a crucial point in our project. </p>
+                            <p>By combining these components with the spray drying method from <a onClick={() => goToPageAndScroll ('rnhale', '/human-practices')}> RNhale </a> [2] we offer a versatile and efficient method for delivering mRNA therapeutics to the lung, paving the way for gene therapy, especially our Prime Guide. The effective delivery of the prime editing complex is a crucial point in our project. </p>
                         <H5 text="Storage" id="text" />
                            <p>The final LNP solution can be stored at 4 °C for a few days. It is recommended to use the formulated LNPs as soon as possible to maintain consistent results. Storage at RT is not recommended. Storage at freezing temperatures is also not recommended unless optimized cryoprotectants are used.</p>