<p>This section highlights the key materials and methods pivotal to advancing our project with the primary goal to develop an efficient prime editing technology to correct the F508del mutation in the CFTR gene by the delivery to lung epithelial cells using optimized lipid nanoparticles (LNPs) via pulmonary administration. We utilized patch clamp electrophysiology to precisely measure ion channel activity, providing crucial insights into cellular function and the impact of genetic modifications on CFTR performance. Additionally, our cell culture models of lung epithelial cells allowed us to test both the delivery and efficacy of our gene-editing system under conditions that closely mimic the <i>in vivo</i> environment. To ensure that our LNPs were both effective and safe, we performed extensive LNP cytotoxicity and characterization experiments, evaluating their biocompatibility, stability, and efficiency in delivering the editing technology. Each of these methodologies was carefully selected to optimize the delivery process and maximize the therapeutic potential of our approach.</p>
