<H3id="Proof of Concept"text="Proof of Concept"></H3>
<p>
To test prime editors, a reliable model system is required. HEK293 cells are a human derived cell line and widely used in a variety of fields in biology<TabScrollLinktab="tab-proof-of-concept"num="1"scrollId="desc-1"/>. Apart from easy handling and comparatively easy transfection, they have, as we found out in our exchange with Mattijs Bulcaen[link], one advantage over other models: They are naturally impaired in DNA repair mechanisms and therefore easier to edit. To properly compare editing efficiencies, a high transfection efficiency is of utmost importance. This engineering cycle focuses on our work in simulating prime editing using the PEAR reporter system<TabScrollLinktab="tab-proof-of-concept"num="2"scrollId="desc-2"/> and optimizing transfection protocols.
To test prime editors, a reliable model system is required. HEK293 cells are a human derived cell line and widely used in a variety of fields in biology<TabScrollLinktab="tab-proof-of-concept"num="1"scrollId="desc-1"/>. Apart from easy handling and comparatively easy transfection, they have, as we found out in our exchange with <aonClick={()=>goToPagesAndOpenTab('mattijsinv','/human-practices')}>Mattijs Bulcaen</a>, one advantage over other models: They are naturally impaired in DNA repair mechanisms and therefore easier to edit. To properly compare editing efficiencies, a high transfection efficiency is of utmost importance. This engineering cycle focuses on our work in simulating prime editing using the PEAR reporter system<TabScrollLinktab="tab-proof-of-concept"num="2"scrollId="desc-2"/> and optimizing transfection protocols.
</p>
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@@ -259,7 +259,7 @@ export function Engineering() {
<H3text="PE6c"id="text"/>
<H4text="Design"id="text"/>
<p>
During our initial talk with Mattijs Bulcaen[Link], he recommended a talk of David Liu[Link] at an online conference, where he presented unpublished data about his laboratory working on prime editing for F508del correction. We investigated it and through this came across the PE6 generation of prime editors. Seeing that the Liu Laboratory eventually decided on using the PE6c system, we adopted the findings.
During our initial talk with <aonClick={()=>goToPagesAndOpenTab('mattijsinv','/human-practices')}>Mattijs Bulcaen</a>, he recommended a talk of <aonClick={()=>goToPagesAndOpenTab('liu','/human-practices')}>David Liu</a> at an online conference, where he presented unpublished data about his laboratory working on prime editing for F508del correction. We investigated it and through this came across the PE6 generation of prime editors. Seeing that the Liu Laboratory eventually decided on using the PE6c system, we adopted the findings.