res

src/contents/results.tsx

@@ -285,14 +285,14 @@ export function Results() {
         <p>While Cryo-EM provides valuable insights into the morphology and size distribution of the LNPs, there are some limitations to this technique. The imaging process involves cryogenic freezing and exposure to high-energy electron beams, which can potentially induce minor structural artifacts. Furthermore, the thinness of the sample may limit contrast, making it difficult to fully distinguish between different LNP populations or their internal structures. Despite these limitations, Cryo-EM still offers a high-resolution view of the LNPs in their near-native state, providing essential information about their size and shape.</p>
         <H4 text="Sort LNP"/>
         <H5 text="Transfection"/>
-          <p>Text :D</p>
+          <p>Already at 48 h of 72 monitored hours post-transfection of HEK293 with the SORT LNP, clear and strong fluorescence was already observed when using LNPs combined with Minicircle DNA, as well as in the Lipofectamine and Minicircle DNA condition, confirming successful transfection and robust expression of the eYFP reporter (Figure 23). This early detection of fluorescence highlights the efficiency of SORT LNPs as delivery system. In contrast, no fluorescence was detected in any conditions involving Lipofectamine alone, Lipofectamine with RNA, or LNPs combined with DNA or RNA, further emphasizing the superior performance of the Minicircle DNA formulations and highlighting the need for improvement for our DNA choice and RNA synthesis.</p>
             <figure>
-              <img src="Insert URL here"/>
+              <img src="https://static.igem.wiki/teams/5247/delivery/results/precyse/sort.png"/>
               <figcaption>
-                <b>Figure X. </b>
-                Description here
+                <b>Figure 23. </b>
+                Overlay of phase contrast and fluorescence microscopic images of transfected HEK293 cells at 20x magnification after 48 h post-transfection with different SORT LNP formulations recorded with Leica DMI6000 B.
               </figcaption>
-            </figure>
+        </figure>
         <H5 text="Flow cytometry"/>
           <div className="row align-items-center">
           <p>We performed flow cytometries analysis 72 h post-transfection to evaluate the transfection efficiency of the SORT LNP in HEK293. The relative percentage of fluorescent cells was determined by measuring the percentage of FITC-A+ cells, followed by normalization to the negative control and fold change calculation.</p>