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Commit 3236d25b authored by Isabell Alexandra Guckes's avatar Isabell Alexandra Guckes
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<p>DMG-PEG (Dimyristoylglycerin-polyethyleneglycol) is an important component by improving the LNP stability and preventing aggregation of the LNPs. </p>
<H4 text="Production Methods" id="text" />
<H5 text="LNP Assembly" id="text" />
<p>Our LNP can be formulated using various methods depending on the scale of production, including pipette mixing, vortex mixing, or microfluidic mixing. After mixing the lipids with mRNA in carefully controlled ratios, the mixture is typically dialyzed to remove organic solvents like ethanol and citrate buffer. The choice of lipid composition and preparation method influences the tissue-targeting capabilities of the LNPs, allowing for selective delivery to organs like the liver, lungs, or spleen. For more detailed information on formulation methods and lipid selection, refer to our LNP Handbook deigned in <a onClick={() => goToPageAndScroll ('handbook', '/human-practices')}> cooperation with iGEM Team Linköping </a> and others.</p>
<p>Our LNP can be formulated using various methods depending on the scale of production, including pipette mixing, vortex mixing, or microfluidic mixing. For cargo protection the mRNA can be mixed with chitosan for stability improvement before adding with the LNP components. After mixing the lipids with mRNA in carefully controlled ratios, the mixture is typically dialyzed to remove organic solvents like ethanol and citrate buffer. The choice of lipid composition and preparation method influences the tissue-targeting capabilities of the LNPs, allowing for selective delivery to organs like the liver, lungs, or spleen. For more detailed information on formulation methods and lipid selection, refer to our LNP Handbook deigned in <a onClick={() => goToPageAndScroll ('handbook', '/human-practices')}> cooperation with iGEM Team Linköping </a> and others.</p>
<p>Click this Button to gain the LNP Handbook</p>
<DownloadLink url="https://static.igem.wiki/teams/5387/liposomes-handbook.pdf" fileName="liposomes-handbook.pdf" />
<H5 text="Spray drying Procedure" id="text" />
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