<H4text="Dynamic Light Scattering (DLS) and Zeta Potential"></H4>
<p>We used dynamic light scattering (DLS) to measure the size distribution and polydispersity index (PDI) of our LNPs. This technique allowed us to confirm that the LNPs had a consistent size distribution with minimal aggregation, which is essential for their stability. Additionally, we measured the zeta potential of the LNPs to assess their surface charge. A high zeta potential confirmed that the LNPs were stable in suspension, which is critical for their effectiveness in biological environments. </p>
<H4text="SEM and Cryo-EM for Structural Analysis"></H4>
<p>To further characterize the morphology and surface structure of the LNPs, we employed scanning electron microscopy (SEM). SEM provided high-resolution images that confirmed the spherical shape and uniformity of the LNPs. Additionally, cryo-transmission electron microscopy (cryo-TEM) allowed us to investigate the internal structure of the LNPs, revealing the presence of lipid layers and encapsulated materials, which are crucial for understanding their function in drug delivery. </p>
<p>To further characterize the morphology and surface structure of the LNPs, we employed scanning electron microscopy (SEM). SEM provided high-resolution images that confirmed the spherical shape and uniformity of the LNPs. Additionally, cryo-electron microscopy (cryo-EM) allowed us to investigate the internal structure of the LNPs, revealing the presence of lipid layers and encapsulated materials, which are crucial for understanding their function in drug delivery. </p>
{/* <H4 text="DNase Assay for Stability of Encapsulated Material "></H4>
<p>Finally, we conducted a DNase assay to evaluate whether the LNPs could protect encapsulated nucleic acids, such as mRNA, from enzymatic degradation. This assay demonstrated that the LNPs successfully shielded the genetic material, ensuring its stability until it reaches target cells. </p> */}
