<br>The fragments SUMO and HSP60-pET-backbone were successfully amplified through PCR and then joined to form plasmid SUMO-HSP60 through homologous recombination. Ultimately, we successfully transferred the plasmid into DH5α and BL21 (DE3) (See more in <astyle="font-family: 'Montserrat';color:#e1a965;text-decoration:underline dotted #e1a965;text-align:right;font-size:inherit;font-weight: inherit;"href="{{ url_for('pages', page='results')}}">
<br>The fragments SUMO and HSP60-pET-backbone were successfully amplified through PCR and then joined to form plasmid SUMO-HSP60 through homologous recombination. Ultimately, we successfully transferred the plasmid into <i>E. coli</i>DH5α and BL21 (DE3) (See more in <astyle="font-family: 'Montserrat';color:#e1a965;text-decoration:underline dotted #e1a965;text-align:right;font-size:inherit;font-weight: inherit;"href="{{ url_for('pages', page='results')}}">
Results page</a>). Moreover, we successfully amplified LAP and HSP60 segments for the backbone of pBAD, yet with the change of GST tag and further optimization (mentioned below) they couldn't be employed anymore.