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Commit b869cb12 authored by raven233's avatar raven233
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<p>
<b>● Test</b>
<br>The fragments SUMO and HSP60-pET-backbone were successfully amplified through PCR and then joined to form plasmid SUMO-HSP60 through homologous recombination. Ultimately, we successfully transferred the plasmid into DH5α and BL21 (DE3) (See more in <a style="font-family: 'Montserrat';color:#e1a965;text-decoration:underline dotted #e1a965;text-align:right;font-size:inherit;font-weight: inherit;"href="{{ url_for('pages', page='results')}}">
Result page</a>). Moreover, we successfully amplified LAP and HSP60 segments for the backbone of pBAD, yet with the change of GST tag and further optimization (mentioned below) they couldn't be employed anymore.
Results page</a>). Moreover, we successfully amplified LAP and HSP60 segments for the backbone of pBAD, yet with the change of GST tag and further optimization (mentioned below) they couldn't be employed anymore.
</p>
<img class="figu enlargeable" style="width:40%; left: 30%;position:relative;" src="https://static.igem.wiki/teams/5048/engineering-adhesion-fig6.webp" >
<figcaption style="text-align: center;"><span class="fig">Fig. 19</span><span class="figt"> The agarose gel electrophoresis of LAP and HSP60 segments PCR products. LAP: 2654bp; HSP60: 1816bp.
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