<h4class="text-center mb-4">Types of organisms used in our project and their potential risks</h4>
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<p>Synthetic biology will need to be used safely and securely if local people are to solve local problems all around the world. The Safety and Security Committee is challenging teams to apply biological engineering approaches to manage risks associated with synthetic biology. Can you take the next step in incremental progress towards knowledge, understanding, and tools that will make the use of synthetic biology safer and more secure?</p>
<p>To compete for the Safety and Security award, please describe your work on this page and also fill out the description on the <ahref="https://competition.igem.org/deliverables/judging-form">judging form</a>.</p>
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<p>Please see the <ahref="https://competition.igem.org/judging/awards">2024 Awards Page</a> for more information.</p>
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<li><i>E. coli</i> DH5-alpha</li>
<li><i>E. coli</i> BL21 (DE3)</li>
<li><i>E. coli</i> RR1</li>
<li><i>S. cerevisiae</i> W303-1A</li>
<li><i>S. cerevisiae</i> @303-1B</li>
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<pclass="mt-3">As the main organisms used are non-pathogenic bacteria, or induce very mild symptoms, if these microorganisms are ingested, they do not pose any real risk to the environment, if correct disposal is carried out, or to the members of the team who carried out the experiments.
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<h4>What should this page contain?</h4>
<h4>Biosecurity</h4>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can go beyond the questions on the safety forms, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
<p>Please visit the <ahref="https://responsibility.igem.org/safety-policies/introduction">Safety Policies page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
<p>Because the work as a whole was developed in laboratories on the premises of the University of São Paulo (USP), the biosafety standards pre-established by institutions, such as the Institute of Biomedical Sciences (ICB) were respected. All the wet lab member already did an biosafety course of Microbiology department to work on our labs , as well as the biosafety guidelines established by federal agencies in Brazil, such as CTNBio (Comissão Técnica Nacional de Biossegurança), such as appropriate hygiene measures and correct use of personal protective equipment (PPE).
It is also important to highlight that during the experiments, the biosafety level adopted was 1, due to the classification of the laboratories used. During our experiments, none of the compounds or tools used were high risk, and the genetic sequences, such as plasmids, were all produced by companies, through iGEM partnerships, or were provided through the laboratory itself or collaborators.</p>
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<h4>Safe Project Design</h4>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<li>Choosing a non-pathogenic chassis</li>
<li>Choosing parts that will not harm humans / animals / plants</li>
<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
<li>Including an "induced lethality" or "kill-switch" device</li>
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<h4>Safe Lab Work</h4>
<h4>Ethics</h4>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
<p>Our study is solely related to the editing of bacteria, expression of compounds of interest and the use of computational techniques, such as genomic and protein analysis. Therefore, no approval from the ethics committee was required, as it did not involve vertebrate animals.
