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2024 Competition
UBC-Vancouver
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0d28f9e0
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0d28f9e0
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5 months ago
by
Ada Jiang
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Update file 2024-07-09-gelelectrophoresis.md
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@@ -22,7 +22,6 @@ tags: []
1.
Make 30ml of 1% agarose gel in 1x TAE
1.
Add 3ul of SYBR safe in the liquid when its lukewarm
2.
Fill the running tray with 1x TAE —> ran out of 1x TAE while filling the electrophoresis tank so we added a bit of old 1x TAE from the electrophoresis station
Open to see image of old 1x TAE used

3.
Add loading buffer into the DNA sample(s) (6x loading buffer)
-
For the sample and the control, we took out 5ul from each PCR tube and put it into a new tube, then added 1ul loading buffer to the new tube.
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