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Commit f950b203 authored by Ángel's avatar Ángel
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<div class="pagebody"> <div class="pagebody">
<div class="pagenav"> <div class="pagenav">
<div onclick="goTo(document.getElementById('one'))"><span id="subtitle1" style="color: #62D881;"><strong>DRY LAB</strong></span></div> <div onclick="goTo(document.getElementById('one'))"><span id="subtitle1" style="color: #62D881;"><strong>DRY LAB</strong></span></div>
<div onclick="goTo(document.getElementById('two'))"><span id="subtitle2" style="color: #62D881;">The uTP sequence</div>
<div onclick="goTo(document.getElementById('two'))"><span id="subtitle2" style="color: #62D881;"><strong>WET LAB</strong></span></div> <div onclick="goTo(document.getElementById('three'))"><span id="subtitle3" style="color: #62D881;"><strong>WET LAB</strong></span></div>
<div onclick="goTo(document.getElementById('three'))"><span id="subtitle3" style="color: #62D881;">uTP expression</div> <div onclick="goTo(document.getElementById('four'))"><span id="subtitle4" style="color: #62D881;">uTP expression</div>
<div onclick="goTo(document.getElementById('four'))"><span id="subtitle4" style="color: #62D881;">UCYN-A fusion</div> <div onclick="goTo(document.getElementById('five'))"><span id="subtitle5" style="color: #62D881;">UCYN-A fusion</div>
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<img src="https://static.igem.wiki/teams/5054/graphical-abstract.png" alt="Fig 1: Graphical overview of the experiment plan."> <img src="https://static.igem.wiki/teams/5054/graphical-abstract.png" alt="Fig 1: Graphical overview of the experiment plan.">
<figcaption>Fig 1: Graphical overview of the experiment plan. </figcaption> <figcaption>Fig 1: Graphical overview of the experiment plan. </figcaption>
</div> </div>
</div>
<div class="h2">The UCYN-A transit peptide sequence</div> <div class="h" id="two">
<div class="h2" style="margin-top: 10px">The UCYN-A transit peptide sequence</div>
<p>If, like other organelles, UCYN-A relies on proteins imported from the host for normal functioning, characterizing the import system and the targeting sequence is essential before transplanting the organelle into a new host organism. Building upon the work of Coale et al. [1], we aimed to advance the understanding of uTP by identifying its precise sequence.</p> <p>If, like other organelles, UCYN-A relies on proteins imported from the host for normal functioning, characterizing the import system and the targeting sequence is essential before transplanting the organelle into a new host organism. Building upon the work of Coale et al. [1], we aimed to advance the understanding of uTP by identifying its precise sequence.</p>
<p>Starting from the raw proteomics data from [1], we selected 368 proteins expressed by the host and significantly enriched in UCYN-A and performed multiple sequence alignment (MSA). Using the alignment we identified a strongly conserved C-terminal region in many of the imported proteins similar to that reported by [1]. We selected a subset of 206 proteins with highly similar (>60% sequence identity) C-terminal alignments, indicating that these are likely to contain uTP, </p> <p>Starting from the raw proteomics data from [1], we selected 368 proteins expressed by the host and significantly enriched in UCYN-A and performed multiple sequence alignment (MSA). Using the alignment we identified a strongly conserved C-terminal region in many of the imported proteins similar to that reported by [1]. We selected a subset of 206 proteins with highly similar (>60% sequence identity) C-terminal alignments, indicating that these are likely to contain uTP, </p>
<div class="img-pagestyle"> <div class="img-pagestyle">
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<div class="h" id="two"> <div class="h" id="three">
<div class="h1"><strong>WET LAB</strong></div> <div class="h1"><strong>WET LAB</strong></div>
</div> </div>
<div class="h" id="three"> <div class="h" id="four">
<div class="h2"><em>in vivo</em> uTP characterization</div> <div class="h2"><em>in vivo</em> uTP characterization</div>
<div class="h3"><strong>Expression of uTP tagged by Fluorescent proteins</strong></div> <div class="h3"><strong>Expression of uTP tagged by Fluorescent proteins</strong></div>
<p></p> <p></p>
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<div class="h" id="four"> <div class="h" id="five">
<div class="h2">UCYN-A isolation and fusion</div> <div class="h2">UCYN-A isolation and fusion</div>
<div class="h3"><strong><em>B. bigelowii</em> culture and UCYN-A isolation</strong></div> <div class="h3"><strong><em>B. bigelowii</em> culture and UCYN-A isolation</strong></div>
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