<divclass="h3"><strong>Expression of uTP tagged by Fluorescent proteins</strong></div>
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<divclass="h2">UCYN-A isolation and fusion</div>
<divclass="h3"><em>B. bigelowii</em> culture and UCYN-A isolation</div>
<divclass="h3"><strong><em>B. bigelowii</em> culture and UCYN-A isolation</strong></div>
<p>Thanks to the generous help of Dr. Kyoko Hagino, a pioneer in research into B. bigelowii, we obtained a culture of B. bigelowii FR-21 [1]. This species is known to be difficult to work with, however, we were able to find the optimal conditions and grow it in our lab in Delft, establishing, to our knowledge, the first B. bigelowii culture in Europe. We followed Kyoko’s advice when deciding on our culture conditions, which can be found in our Materials and Methods section.</p>