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Commit c8bdc631 authored by Tabel5's avatar Tabel5
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Merge branch 'main' of gitlab.igem.org:2024/tu-delft

parents f8b8e132 5d4a0180
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static/consensus-c-term.pdb 0 → 100644
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ATOM 1 N SER A 66 -10.867 -1.081 9.220 1.00 72.45 N
ATOM 2 CA SER A 66 -11.819 -0.538 8.249 1.00 75.40 C
ATOM 3 C SER A 66 -11.299 0.718 7.543 1.00 76.19 C
ATOM 4 O SER A 66 -11.516 0.888 6.347 1.00 75.04 O
ATOM 5 CB SER A 66 -13.171 -0.249 8.913 1.00 73.52 C
ATOM 6 OG SER A 66 -13.067 0.724 9.932 1.00 64.46 O
ATOM 7 N SER A 67 -11.392 1.551 8.433 1.00 66.81 N
ATOM 8 CA SER A 67 -10.634 2.559 7.684 1.00 69.62 C
ATOM 9 C SER A 67 -9.536 1.959 6.794 1.00 72.50 C
ATOM 10 O SER A 67 -9.309 2.460 5.696 1.00 70.77 O
ATOM 11 CB SER A 67 -10.054 3.612 8.634 1.00 64.95 C
ATOM 12 OG SER A 67 -9.005 3.117 9.433 1.00 54.96 O
ATOM 13 N GLU A 68 -8.943 1.042 7.328 1.00 68.14 N
ATOM 14 CA GLU A 68 -7.906 0.367 6.550 1.00 69.86 C
ATOM 15 C GLU A 68 -8.493 -0.310 5.297 1.00 71.64 C
ATOM 16 O GLU A 68 -7.973 -0.124 4.196 1.00 71.04 O
ATOM 17 CB GLU A 68 -7.182 -0.624 7.479 1.00 67.92 C
ATOM 18 CG GLU A 68 -5.983 -1.313 6.821 1.00 61.18 C
ATOM 19 CD GLU A 68 -5.225 -2.269 7.758 1.00 58.37 C
ATOM 20 OE1 GLU A 68 -4.239 -2.883 7.273 1.00 55.19 O
ATOM 21 OE2 GLU A 68 -5.599 -2.409 8.948 1.00 53.09 O
ATOM 22 N GLU A 69 -8.938 -0.502 5.109 1.00 67.38 N
ATOM 23 CA GLU A 69 -9.642 -1.108 3.973 1.00 69.26 C
ATOM 24 C GLU A 69 -10.083 -0.060 2.944 1.00 70.83 C
ATOM 25 O GLU A 69 -9.797 -0.220 1.759 1.00 70.40 O
ATOM 26 CB GLU A 69 -10.856 -1.924 4.467 1.00 67.42 C
ATOM 27 CG GLU A 69 -10.436 -3.283 5.047 1.00 58.92 C
ATOM 28 CD GLU A 69 -11.606 -4.092 5.663 1.00 54.49 C
ATOM 29 OE1 GLU A 69 -11.355 -5.263 6.037 1.00 52.25 O
ATOM 30 OE2 GLU A 69 -12.726 -3.557 5.779 1.00 48.82 O
ATOM 31 N ALA A 70 -11.311 0.522 4.101 1.00 73.58 N
ATOM 32 CA ALA A 70 -11.718 1.599 3.202 1.00 74.52 C
ATOM 33 C ALA A 70 -10.529 2.256 2.486 1.00 76.35 C
ATOM 34 O ALA A 70 -10.627 2.598 1.309 1.00 75.85 O
ATOM 35 CB ALA A 70 -12.495 2.628 4.017 1.00 72.57 C
ATOM 36 N TRP A 71 -9.190 2.000 2.779 1.00 77.87 N
ATOM 37 CA TRP A 71 -7.974 2.515 2.164 1.00 79.51 C
ATOM 38 C TRP A 71 -7.446 1.570 1.078 1.00 79.62 C
ATOM 39 O TRP A 71 -7.213 2.033 -0.046 1.00 80.34 O
ATOM 40 CB TRP A 71 -6.923 2.778 3.243 1.00 80.37 C
ATOM 41 CG TRP A 71 -5.665 3.418 2.732 1.00 79.99 C
ATOM 42 CD1 TRP A 71 -5.387 4.741 2.742 1.00 79.42 C
ATOM 43 CD2 TRP A 71 -4.510 2.771 2.116 1.00 80.19 C
ATOM 44 CE2 TRP A 71 -3.568 3.786 1.780 1.00 79.92 C
ATOM 45 CE3 TRP A 71 -4.165 1.439 1.812 1.00 79.52 C
ATOM 46 NE1 TRP A 71 -4.155 4.963 2.193 1.00 79.29 N
ATOM 47 CZ2 TRP A 71 -2.334 3.484 1.154 1.00 78.18 C
ATOM 48 CZ3 TRP A 71 -2.936 1.132 1.189 1.00 78.51 C
ATOM 49 CH2 TRP A 71 -2.030 2.145 0.861 1.00 77.42 C
ATOM 50 N LEU A 72 -7.317 -0.061 1.569 1.00 76.93 N
ATOM 51 CA LEU A 72 -6.866 -1.079 0.617 1.00 76.65 C
ATOM 52 C LEU A 72 -7.771 -1.140 -0.615 1.00 77.62 C
ATOM 53 O LEU A 72 -7.273 -1.121 -1.738 1.00 77.56 O
ATOM 54 CB LEU A 72 -6.835 -2.453 1.313 1.00 75.03 C
ATOM 55 CG LEU A 72 -5.631 -2.670 2.239 1.00 68.68 C
ATOM 56 CD1 LEU A 72 -5.824 -3.949 3.050 1.00 63.91 C
ATOM 57 CD2 LEU A 72 -4.326 -2.811 1.445 1.00 63.23 C
ATOM 58 N GLU A 73 -8.629 -0.315 -1.015 1.00 68.26 N
ATOM 59 CA GLU A 73 -9.637 -0.220 -2.072 1.00 69.50 C
ATOM 60 C GLU A 73 -9.405 1.016 -2.950 1.00 70.59 C
ATOM 61 O GLU A 73 -9.381 0.907 -4.179 1.00 70.11 O
ATOM 62 CB GLU A 73 -11.051 -0.176 -1.475 1.00 67.24 C
ATOM 63 CG GLU A 73 -11.521 -1.551 -0.961 1.00 59.67 C
ATOM 64 CD GLU A 73 -12.959 -1.533 -0.404 1.00 55.03 C
ATOM 65 OE1 GLU A 73 -13.479 -2.641 -0.111 1.00 51.73 O
ATOM 66 OE2 GLU A 73 -13.573 -0.444 -0.319 1.00 48.54 O
ATOM 67 N GLU A 74 -10.050 1.004 -2.238 1.00 72.43 N
ATOM 68 CA GLU A 74 -9.757 2.235 -2.973 1.00 74.18 C
ATOM 69 C GLU A 74 -8.451 2.116 -3.773 1.00 75.21 C
ATOM 70 O GLU A 74 -8.418 2.455 -4.960 1.00 74.72 O
ATOM 71 CB GLU A 74 -9.718 3.421 -2.002 1.00 73.00 C
ATOM 72 CG GLU A 74 -9.567 4.734 -2.781 1.00 67.29 C
ATOM 73 CD GLU A 74 -9.542 5.992 -1.907 1.00 66.64 C
ATOM 74 OE1 GLU A 74 -9.279 7.060 -2.512 1.00 60.94 O
ATOM 75 OE2 GLU A 74 -9.733 5.915 -0.684 1.00 61.07 O
ATOM 76 N TRP A 75 -7.280 2.232 -2.709 1.00 79.54 N
ATOM 77 CA TRP A 75 -5.989 2.063 -3.357 1.00 81.57 C
ATOM 78 C TRP A 75 -6.069 1.091 -4.533 1.00 81.89 C
ATOM 79 O TRP A 75 -5.646 1.444 -5.633 1.00 81.70 O
ATOM 80 CB TRP A 75 -4.949 1.608 -2.330 1.00 83.08 C
ATOM 81 CG TRP A 75 -3.648 1.193 -2.943 1.00 84.30 C
ATOM 82 CD1 TRP A 75 -3.101 -0.043 -2.892 1.00 82.84 C
ATOM 83 CD2 TRP A 75 -2.762 1.988 -3.795 1.00 83.99 C
ATOM 84 CE2 TRP A 75 -1.691 1.157 -4.221 1.00 83.35 C
ATOM 85 CE3 TRP A 75 -2.776 3.321 -4.252 1.00 83.00 C
ATOM 86 NE1 TRP A 75 -1.945 -0.061 -3.623 1.00 82.65 N
ATOM 87 CZ2 TRP A 75 -0.675 1.620 -5.073 1.00 82.61 C
ATOM 88 CZ3 TRP A 75 -1.752 3.794 -5.108 1.00 82.11 C
ATOM 89 CH2 TRP A 75 -0.714 2.943 -5.517 1.00 80.84 C
ATOM 90 N ARG A 76 -5.886 0.827 -5.437 1.00 78.96 N
ATOM 91 CA ARG A 76 -6.279 -0.107 -6.503 1.00 78.10 C
ATOM 92 C ARG A 76 -7.076 0.585 -7.605 1.00 80.49 C
ATOM 93 O ARG A 76 -6.814 0.337 -8.775 1.00 81.00 O
ATOM 94 CB ARG A 76 -7.085 -1.285 -5.929 1.00 73.50 C
ATOM 95 CG ARG A 76 -6.196 -2.292 -5.193 1.00 65.03 C
ATOM 96 CD ARG A 76 -6.987 -3.522 -4.712 1.00 60.95 C
ATOM 97 NE ARG A 76 -8.018 -3.168 -3.715 1.00 56.66 N
ATOM 98 CZ ARG A 76 -8.598 -4.005 -2.871 1.00 48.85 C
ATOM 99 NH1 ARG A 76 -8.306 -5.277 -2.825 1.00 47.77 N
ATOM 100 NH2 ARG A 76 -9.507 -3.585 -2.025 1.00 46.57 N
ATOM 101 N GLU A 77 -8.250 0.455 -6.893 1.00 84.10 N
ATOM 102 CA GLU A 77 -9.018 1.039 -8.000 1.00 85.16 C
ATOM 103 C GLU A 77 -8.128 1.880 -8.923 1.00 86.47 C
ATOM 104 O GLU A 77 -8.178 1.731 -10.152 1.00 86.59 O
ATOM 105 CB GLU A 77 -10.134 1.936 -7.449 1.00 84.51 C
ATOM 106 CG GLU A 77 -11.273 1.152 -6.787 1.00 76.94 C
ATOM 107 CD GLU A 77 -12.391 2.088 -6.283 1.00 71.70 C
ATOM 108 OE1 GLU A 77 -13.502 1.567 -6.022 1.00 64.41 O
ATOM 109 OE2 GLU A 77 -12.158 3.321 -6.233 1.00 64.41 O
ATOM 110 N ARG A 78 -7.508 2.459 -7.956 1.00 83.57 N
ATOM 111 CA ARG A 78 -6.597 3.411 -8.620 1.00 84.67 C
ATOM 112 C ARG A 78 -5.545 2.703 -9.465 1.00 85.62 C
ATOM 113 O ARG A 78 -5.310 3.098 -10.601 1.00 85.22 O
ATOM 114 CB ARG A 78 -5.905 4.309 -7.577 1.00 84.12 C
ATOM 115 CG ARG A 78 -6.851 5.342 -6.969 1.00 80.91 C
ATOM 116 CD ARG A 78 -6.121 6.101 -5.846 1.00 79.09 C
ATOM 117 NE ARG A 78 -7.015 7.008 -5.124 1.00 74.53 N
ATOM 118 CZ ARG A 78 -6.675 8.159 -4.549 1.00 70.16 C
ATOM 119 NH1 ARG A 78 -5.463 8.625 -4.582 1.00 66.18 N
ATOM 120 NH2 ARG A 78 -7.571 8.853 -3.907 1.00 65.87 N
ATOM 121 N LEU A 79 -5.195 1.813 -8.661 1.00 81.83 N
ATOM 122 CA LEU A 79 -4.181 1.031 -9.357 1.00 82.36 C
ATOM 123 C LEU A 79 -4.775 0.291 -10.561 1.00 82.53 C
ATOM 124 O LEU A 79 -4.180 0.299 -11.628 1.00 80.76 O
ATOM 125 CB LEU A 79 -3.516 0.062 -8.354 1.00 81.63 C
ATOM 126 CG LEU A 79 -2.290 -0.681 -8.917 1.00 78.84 C
ATOM 127 CD1 LEU A 79 -1.112 0.267 -9.178 1.00 74.67 C
ATOM 128 CD2 LEU A 79 -1.837 -1.743 -7.919 1.00 74.10 C
ATOM 129 N GLU A 80 -5.529 1.079 -11.340 1.00 79.78 N
ATOM 130 CA GLU A 80 -6.270 0.429 -12.427 1.00 81.06 C
ATOM 131 C GLU A 80 -6.646 1.416 -13.531 1.00 81.20 C
ATOM 132 O GLU A 80 -6.440 1.135 -14.710 1.00 79.63 O
ATOM 133 CB GLU A 80 -7.536 -0.226 -11.868 1.00 77.82 C
ATOM 134 CG GLU A 80 -7.252 -1.514 -11.082 1.00 71.51 C
ATOM 135 CD GLU A 80 -8.509 -2.065 -10.381 1.00 65.85 C
ATOM 136 OE1 GLU A 80 -8.352 -3.024 -9.591 1.00 60.62 O
ATOM 137 OE2 GLU A 80 -9.621 -1.549 -10.643 1.00 59.24 O
ATOM 138 N CYS A 81 -7.259 1.338 -12.294 1.00 79.14 N
ATOM 139 CA CYS A 81 -7.542 2.375 -13.292 1.00 80.93 C
ATOM 140 C CYS A 81 -6.272 2.865 -14.002 1.00 82.05 C
ATOM 141 O CYS A 81 -6.270 3.019 -15.218 1.00 78.88 O
ATOM 142 CB CYS A 81 -8.256 3.559 -12.627 1.00 78.16 C
ATOM 143 SG CYS A 81 -9.992 3.281 -12.206 1.00 71.78 S
ATOM 144 N TRP A 82 -5.371 2.792 -13.145 1.00 85.26 N
ATOM 145 CA TRP A 82 -4.086 3.166 -13.724 1.00 86.80 C
ATOM 146 C TRP A 82 -3.560 2.077 -14.664 1.00 87.12 C
ATOM 147 O TRP A 82 -3.177 2.390 -15.792 1.00 86.38 O
ATOM 148 CB TRP A 82 -3.086 3.453 -12.598 1.00 87.60 C
ATOM 149 CG TRP A 82 -1.662 3.582 -13.054 1.00 88.13 C
ATOM 150 CD1 TRP A 82 -1.082 4.685 -13.567 1.00 87.47 C
ATOM 151 CD2 TRP A 82 -0.642 2.535 -13.081 1.00 88.80 C
ATOM 152 CE2 TRP A 82 0.544 3.091 -13.635 1.00 88.47 C
ATOM 153 CE3 TRP A 82 -0.611 1.177 -12.695 1.00 87.90 C
ATOM 154 NE1 TRP A 82 0.220 4.403 -13.912 1.00 87.53 N
ATOM 155 CZ2 TRP A 82 1.717 2.325 -13.805 1.00 86.71 C
ATOM 156 CZ3 TRP A 82 0.565 0.408 -12.861 1.00 86.98 C
ATOM 157 CH2 TRP A 82 1.713 0.982 -13.413 1.00 85.73 C
ATOM 158 N TRP A 83 -4.003 1.040 -14.659 1.00 73.76 N
ATOM 159 CA TRP A 83 -3.588 -0.164 -15.367 1.00 73.88 C
ATOM 160 C TRP A 83 -4.271 -0.305 -16.730 1.00 72.96 C
ATOM 161 O TRP A 83 -3.630 -0.696 -17.701 1.00 71.24 O
ATOM 162 CB TRP A 83 -3.893 -1.384 -14.495 1.00 74.13 C
ATOM 163 CG TRP A 83 -3.395 -2.673 -15.065 1.00 74.62 C
ATOM 164 CD1 TRP A 83 -4.115 -3.551 -15.796 1.00 69.88 C
ATOM 165 CD2 TRP A 83 -2.051 -3.212 -14.978 1.00 71.66 C
ATOM 166 CE2 TRP A 83 -2.022 -4.449 -15.683 1.00 68.86 C
ATOM 167 CE3 TRP A 83 -0.856 -2.781 -14.371 1.00 70.37 C
ATOM 168 NE1 TRP A 83 -3.308 -4.608 -16.161 1.00 67.17 N
ATOM 169 CZ2 TRP A 83 -0.856 -5.224 -15.771 1.00 70.88 C
ATOM 170 CZ3 TRP A 83 0.315 -3.557 -14.455 1.00 70.17 C
ATOM 171 CH2 TRP A 83 0.314 -4.765 -15.150 1.00 69.16 C
ATOM 172 N LEU A 84 -5.576 -0.153 -16.138 1.00 68.14 N
ATOM 173 CA LEU A 84 -6.315 -0.148 -17.399 1.00 66.04 C
ATOM 174 C LEU A 84 -5.848 0.961 -18.354 1.00 66.25 C
ATOM 175 O LEU A 84 -5.893 0.790 -19.570 1.00 63.64 O
ATOM 176 CB LEU A 84 -7.820 0.004 -17.100 1.00 63.51 C
ATOM 177 CG LEU A 84 -8.457 -1.206 -16.382 1.00 58.55 C
ATOM 178 CD1 LEU A 84 -9.894 -0.866 -15.995 1.00 54.23 C
ATOM 179 CD2 LEU A 84 -8.469 -2.451 -17.260 1.00 53.12 C
ATOM 180 N GLY A 85 -5.530 2.347 -17.807 1.00 85.35 N
ATOM 181 CA GLY A 85 -5.108 3.478 -18.626 1.00 85.05 C
ATOM 182 C GLY A 85 -3.764 3.289 -19.323 1.00 86.01 C
ATOM 183 O GLY A 85 -3.361 4.167 -20.078 1.00 83.19 O
ATOM 184 N LEU A 86 -2.858 2.005 -19.103 1.00 84.27 N
ATOM 185 CA LEU A 86 -1.580 1.811 -19.785 1.00 85.30 C
ATOM 186 C LEU A 86 -1.769 1.194 -21.181 1.00 86.32 C
ATOM 187 O LEU A 86 -2.660 0.364 -21.384 1.00 85.86 O
ATOM 188 CB LEU A 86 -0.637 0.941 -18.940 1.00 85.55 C
ATOM 189 CG LEU A 86 -0.205 1.571 -17.610 1.00 85.05 C
ATOM 190 CD1 LEU A 86 0.614 0.535 -16.832 1.00 81.13 C
ATOM 191 CD2 LEU A 86 0.666 2.814 -17.800 1.00 80.94 C
ATOM 192 N PRO A 87 -0.767 1.651 -22.122 1.00 86.97 N
ATOM 193 CA PRO A 87 -0.735 0.960 -23.406 1.00 87.26 C
ATOM 194 C PRO A 87 -0.526 -0.554 -23.238 1.00 87.77 C
ATOM 195 O PRO A 87 0.265 -0.979 -22.391 1.00 86.54 O
ATOM 196 CB PRO A 87 0.418 1.601 -24.190 1.00 85.62 C
ATOM 197 CG PRO A 87 0.594 2.978 -23.536 1.00 83.29 C
ATOM 198 CD PRO A 87 0.236 2.706 -22.079 1.00 85.27 C
ATOM 199 N LEU A 88 -0.707 -1.378 -23.756 1.00 83.00 N
ATOM 200 CA LEU A 88 -0.599 -2.848 -23.710 1.00 83.50 C
ATOM 201 C LEU A 88 0.854 -3.380 -23.653 1.00 84.46 C
ATOM 202 O LEU A 88 1.109 -4.264 -22.834 1.00 83.69 O
ATOM 203 CB LEU A 88 -1.366 -3.481 -24.890 1.00 81.46 C
ATOM 204 CG LEU A 88 -2.900 -3.371 -24.810 1.00 73.12 C
ATOM 205 CD1 LEU A 88 -3.506 -3.876 -26.125 1.00 65.79 C
ATOM 206 CD2 LEU A 88 -3.483 -4.211 -23.671 1.00 67.18 C
ATOM 207 N PRO A 89 1.540 -2.962 -24.548 1.00 87.25 N
ATOM 208 CA PRO A 89 2.930 -3.427 -24.460 1.00 87.64 C
ATOM 209 C PRO A 89 3.543 -3.202 -23.070 1.00 87.72 C
ATOM 210 O PRO A 89 4.280 -4.039 -22.569 1.00 86.50 O
ATOM 211 CB PRO A 89 3.694 -2.649 -25.537 1.00 87.39 C
ATOM 212 CG PRO A 89 2.617 -2.251 -26.536 1.00 84.22 C
ATOM 213 CD PRO A 89 1.408 -2.003 -25.635 1.00 86.46 C
ATOM 214 N THR A 90 2.994 -1.950 -22.634 1.00 88.06 N
ATOM 215 CA THR A 90 3.473 -1.632 -21.279 1.00 87.33 C
ATOM 216 C THR A 90 2.814 -2.545 -20.249 1.00 87.46 C
ATOM 217 O THR A 90 3.483 -3.015 -19.329 1.00 86.66 O
ATOM 218 CB THR A 90 3.206 -0.164 -20.935 1.00 86.57 C
ATOM 219 CG2 THR A 90 3.894 0.273 -19.646 1.00 81.93 C
ATOM 220 OG1 THR A 90 3.678 0.672 -21.958 1.00 83.57 O
ATOM 221 N GLN A 91 1.573 -2.821 -20.372 1.00 81.00 N
ATOM 222 CA GLN A 91 0.886 -3.786 -19.507 1.00 80.18 C
ATOM 223 C GLN A 91 1.522 -5.175 -19.598 1.00 80.14 C
ATOM 224 O GLN A 91 1.740 -5.818 -18.571 1.00 78.97 O
ATOM 225 CB GLN A 91 -0.607 -3.886 -19.875 1.00 79.46 C
ATOM 226 CG GLN A 91 -1.405 -2.597 -19.638 1.00 77.04 C
ATOM 227 CD GLN A 91 -2.881 -2.772 -19.992 1.00 76.88 C
ATOM 228 NE2 GLN A 91 -3.582 -1.711 -20.298 1.00 66.59 N
ATOM 229 OE1 GLN A 91 -3.430 -3.866 -20.009 1.00 70.57 O
ATOM 230 N THR A 92 1.896 -5.550 -20.948 1.00 84.41 N
ATOM 231 CA THR A 92 2.545 -6.858 -21.116 1.00 83.22 C
ATOM 232 C THR A 92 3.946 -6.893 -20.501 1.00 82.25 C
ATOM 233 O THR A 92 4.291 -7.873 -19.843 1.00 80.88 O
ATOM 234 CB THR A 92 2.625 -7.292 -22.587 1.00 83.79 C
ATOM 235 CG2 THR A 92 1.240 -7.575 -23.166 1.00 74.76 C
ATOM 236 OG1 THR A 92 3.221 -6.319 -23.400 1.00 77.43 O
ATOM 237 N GLN A 93 4.876 -5.891 -20.122 1.00 86.69 N
ATOM 238 CA GLN A 93 6.183 -5.765 -19.467 1.00 85.77 C
ATOM 239 C GLN A 93 6.050 -5.740 -17.935 1.00 85.33 C
ATOM 240 O GLN A 93 6.775 -6.450 -17.237 1.00 84.82 O
ATOM 241 CB GLN A 93 6.889 -4.498 -19.959 1.00 85.54 C
ATOM 242 CG GLN A 93 7.389 -4.633 -21.404 1.00 79.55 C
ATOM 243 CD GLN A 93 7.958 -3.321 -21.949 1.00 76.54 C
ATOM 244 NE2 GLN A 93 8.367 -3.283 -23.199 1.00 65.84 N
ATOM 245 OE1 GLN A 93 8.055 -2.303 -21.282 1.00 69.14 O
ATOM 246 N LEU A 94 5.130 -4.801 -17.716 1.00 85.15 N
ATOM 247 CA LEU A 94 4.863 -4.775 -16.279 1.00 84.96 C
ATOM 248 C LEU A 94 4.397 -6.124 -15.740 1.00 84.32 C
ATOM 249 O LEU A 94 4.808 -6.493 -14.646 1.00 82.36 O
ATOM 250 CB LEU A 94 3.822 -3.688 -15.960 1.00 84.50 C
ATOM 251 CG LEU A 94 4.408 -2.271 -15.928 1.00 82.43 C
ATOM 252 CD1 LEU A 94 3.267 -1.268 -15.816 1.00 77.86 C
ATOM 253 CD2 LEU A 94 5.332 -2.062 -14.724 1.00 77.82 C
ATOM 254 N GLY A 95 3.245 -6.817 -16.403 1.00 77.97 N
ATOM 255 CA GLY A 95 2.951 -8.239 -16.215 1.00 75.60 C
ATOM 256 C GLY A 95 4.226 -9.072 -16.087 1.00 75.85 C
ATOM 257 O GLY A 95 4.339 -9.875 -15.165 1.00 73.32 O
ATOM 258 N GLY A 96 5.661 -9.051 -17.380 1.00 76.94 N
ATOM 259 CA GLY A 96 6.966 -9.706 -17.251 1.00 77.83 C
ATOM 260 C GLY A 96 7.755 -9.259 -16.015 1.00 78.81 C
ATOM 261 O GLY A 96 8.521 -10.040 -15.450 1.00 74.08 O
ATOM 262 N CYS A 97 7.470 -7.898 -14.928 1.00 84.33 N
ATOM 263 CA CYS A 97 8.194 -7.268 -13.827 1.00 85.52 C
ATOM 264 C CYS A 97 7.316 -6.994 -12.585 1.00 86.00 C
ATOM 265 O CYS A 97 7.639 -6.120 -11.781 1.00 84.54 O
ATOM 266 CB CYS A 97 8.878 -6.000 -14.359 1.00 85.98 C
ATOM 267 SG CYS A 97 10.211 -6.296 -15.562 1.00 85.58 S
ATOM 268 N MET A 98 6.738 -8.215 -12.141 1.00 80.92 N
ATOM 269 CA MET A 98 5.792 -7.981 -11.035 1.00 80.55 C
ATOM 270 C MET A 98 6.443 -8.076 -9.649 1.00 81.50 C
ATOM 271 O MET A 98 6.094 -7.315 -8.751 1.00 77.45 O
ATOM 272 CB MET A 98 4.617 -8.964 -11.115 1.00 76.39 C
ATOM 273 CG MET A 98 3.571 -8.548 -12.147 1.00 69.71 C
ATOM 274 SD MET A 98 2.752 -6.954 -11.871 1.00 64.41 S
ATOM 275 CE MET A 98 1.806 -7.312 -10.368 1.00 56.79 C
ATOM 276 N GLY A 99 6.933 -8.563 -9.898 1.00 78.71 N
ATOM 277 CA GLY A 99 7.707 -8.663 -8.660 1.00 79.41 C
ATOM 278 C GLY A 99 8.518 -7.391 -8.377 1.00 80.82 C
ATOM 279 O GLY A 99 8.453 -6.856 -7.277 1.00 79.28 O
ATOM 280 N ALA A 100 9.291 -6.833 -9.222 1.00 83.38 N
ATOM 281 CA ALA A 100 10.038 -5.583 -9.123 1.00 84.52 C
ATOM 282 C ALA A 100 9.125 -4.383 -8.843 1.00 85.03 C
ATOM 283 O ALA A 100 9.443 -3.545 -7.998 1.00 84.76 O
ATOM 284 CB ALA A 100 10.830 -5.384 -10.420 1.00 84.53 C
ATOM 285 N LEU A 101 7.787 -4.465 -9.557 1.00 84.23 N
ATOM 286 CA LEU A 101 6.777 -3.444 -9.297 1.00 83.90 C
ATOM 287 C LEU A 101 6.284 -3.501 -7.847 1.00 83.70 C
ATOM 288 O LEU A 101 6.185 -2.466 -7.195 1.00 83.20 O
ATOM 289 CB LEU A 101 5.616 -3.617 -10.296 1.00 84.03 C
ATOM 290 CG LEU A 101 4.504 -2.567 -10.139 1.00 82.34 C
ATOM 291 CD1 LEU A 101 4.996 -1.148 -10.447 1.00 78.35 C
ATOM 292 CD2 LEU A 101 3.349 -2.884 -11.082 1.00 77.65 C
ATOM 293 N GLY A 102 6.039 -4.789 -7.302 1.00 81.32 N
ATOM 294 CA GLY A 102 5.629 -4.988 -5.916 1.00 79.77 C
ATOM 295 C GLY A 102 6.665 -4.478 -4.915 1.00 80.19 C
ATOM 296 O GLY A 102 6.319 -3.714 -4.013 1.00 78.46 O
ATOM 297 N LEU A 103 7.987 -4.453 -5.179 1.00 81.16 N
ATOM 298 CA LEU A 103 9.090 -3.965 -4.349 1.00 81.08 C
ATOM 299 C LEU A 103 9.234 -2.439 -4.429 1.00 81.20 C
ATOM 300 O LEU A 103 9.352 -1.775 -3.398 1.00 80.70 O
ATOM 301 CB LEU A 103 10.401 -4.647 -4.777 1.00 80.25 C
ATOM 302 CG LEU A 103 10.483 -6.153 -4.452 1.00 75.97 C
ATOM 303 CD1 LEU A 103 11.749 -6.743 -5.073 1.00 71.74 C
ATOM 304 CD2 LEU A 103 10.511 -6.426 -2.948 1.00 71.86 C
ATOM 305 N HIS A 104 8.805 -2.117 -5.684 1.00 84.05 N
ATOM 306 CA HIS A 104 8.885 -0.674 -5.873 1.00 84.36 C
ATOM 307 C HIS A 104 7.739 0.079 -5.178 1.00 84.37 C
ATOM 308 O HIS A 104 7.966 1.082 -4.497 1.00 83.94 O
ATOM 309 CB HIS A 104 8.908 -0.377 -7.382 1.00 84.61 C
ATOM 310 CG HIS A 104 9.114 1.093 -7.653 1.00 82.80 C
ATOM 311 CD2 HIS A 104 8.218 1.977 -8.195 1.00 75.57 C
ATOM 312 ND1 HIS A 104 10.257 1.805 -7.352 1.00 75.74 N
ATOM 313 CE1 HIS A 104 10.044 3.086 -7.717 1.00 75.92 C
ATOM 314 NE2 HIS A 104 8.821 3.234 -8.227 1.00 77.32 N
ATOM 315 N LEU A 105 6.651 -0.288 -5.185 1.00 79.23 N
ATOM 316 CA LEU A 105 5.492 0.158 -4.422 1.00 79.19 C
ATOM 317 C LEU A 105 5.742 0.102 -2.911 1.00 78.35 C
ATOM 318 O LEU A 105 5.433 1.060 -2.204 1.00 76.99 O
ATOM 319 CB LEU A 105 4.262 -0.690 -4.794 1.00 79.05 C
ATOM 320 CG LEU A 105 3.686 -0.427 -6.195 1.00 76.56 C
ATOM 321 CD1 LEU A 105 2.500 -1.366 -6.415 1.00 72.14 C
ATOM 322 CD2 LEU A 105 3.192 1.009 -6.357 1.00 71.42 C
ATOM 323 N GLY A 106 5.998 -1.288 -2.578 1.00 76.41 N
ATOM 324 CA GLY A 106 6.375 -1.448 -1.176 1.00 74.13 C
ATOM 325 C GLY A 106 7.342 -0.362 -0.701 1.00 75.06 C
ATOM 326 O GLY A 106 7.120 0.246 0.344 1.00 74.15 O
ATOM 327 N SER A 107 8.295 -0.225 -1.484 1.00 80.26 N
ATOM 328 CA SER A 107 9.377 0.691 -1.104 1.00 79.92 C
ATOM 329 C SER A 107 8.908 2.148 -1.070 1.00 79.94 C
ATOM 330 O SER A 107 9.174 2.890 -0.122 1.00 79.67 O
ATOM 331 CB SER A 107 10.552 0.510 -2.071 1.00 79.88 C
ATOM 332 OG SER A 107 11.602 1.379 -1.738 1.00 70.76 O
ATOM 333 N ARG A 108 8.158 2.458 -1.748 1.00 81.81 N
ATOM 334 CA ARG A 108 7.556 3.799 -1.760 1.00 82.29 C
ATOM 335 C ARG A 108 6.563 4.001 -0.616 1.00 82.34 C
ATOM 336 O ARG A 108 6.557 5.079 -0.015 1.00 81.75 O
ATOM 337 CB ARG A 108 6.859 4.072 -3.093 1.00 82.26 C
ATOM 338 CG ARG A 108 7.864 4.328 -4.220 1.00 77.62 C
ATOM 339 CD ARG A 108 7.139 4.738 -5.515 1.00 76.25 C
ATOM 340 NE ARG A 108 6.532 6.083 -5.385 1.00 70.82 N
ATOM 341 CZ ARG A 108 5.473 6.545 -6.038 1.00 66.37 C
ATOM 342 NH1 ARG A 108 4.818 5.835 -6.896 1.00 63.19 N
ATOM 343 NH2 ARG A 108 5.038 7.754 -5.825 1.00 59.78 N
ATOM 344 N LEU A 109 5.661 2.669 -0.348 1.00 81.06 N
ATOM 345 CA LEU A 109 4.694 2.709 0.747 1.00 80.44 C
ATOM 346 C LEU A 109 5.402 2.848 2.093 1.00 80.06 C
ATOM 347 O LEU A 109 5.104 3.781 2.840 1.00 79.22 O
ATOM 348 CB LEU A 109 3.822 1.442 0.675 1.00 80.22 C
ATOM 349 CG LEU A 109 2.712 1.400 1.734 1.00 77.37 C
ATOM 350 CD1 LEU A 109 1.709 2.546 1.556 1.00 74.54 C
ATOM 351 CD2 LEU A 109 1.967 0.072 1.623 1.00 73.32 C
ATOM 352 N ASP A 110 6.292 1.944 2.001 1.00 78.33 N
ATOM 353 CA ASP A 110 7.121 2.000 3.208 1.00 78.04 C
ATOM 354 C ASP A 110 7.829 3.363 3.341 1.00 78.32 C
ATOM 355 O ASP A 110 7.793 4.005 4.397 1.00 78.13 O
ATOM 356 CB ASP A 110 8.133 0.852 3.149 1.00 76.88 C
ATOM 357 CG ASP A 110 9.050 0.908 4.367 1.00 70.55 C
ATOM 358 OD1 ASP A 110 8.546 0.725 5.502 1.00 65.47 O
ATOM 359 OD2 ASP A 110 10.235 1.235 4.166 1.00 63.56 O
ATOM 360 N ALA A 111 8.657 4.379 2.135 1.00 75.17 N
ATOM 361 CA ALA A 111 9.128 5.761 2.135 1.00 75.51 C
ATOM 362 C ALA A 111 8.089 6.735 2.716 1.00 75.24 C
ATOM 363 O ALA A 111 8.447 7.612 3.500 1.00 74.10 O
ATOM 364 CB ALA A 111 9.529 6.144 0.703 1.00 75.30 C
ATOM 365 N SER A 112 6.591 6.577 2.412 1.00 78.79 N
ATOM 366 CA SER A 112 5.537 7.560 2.691 1.00 78.34 C
ATOM 367 C SER A 112 5.074 7.525 4.155 1.00 77.40 C
ATOM 368 O SER A 112 4.889 8.574 4.771 1.00 76.44 O
ATOM 369 CB SER A 112 4.357 7.390 1.723 1.00 78.60 C
ATOM 370 OG SER A 112 3.548 6.279 2.020 1.00 68.98 O
ATOM 371 N LEU A 113 5.129 6.224 4.418 1.00 70.43 N
ATOM 372 CA LEU A 113 5.053 5.875 5.837 1.00 69.40 C
ATOM 373 C LEU A 113 6.233 6.449 6.632 1.00 68.99 C
ATOM 374 O LEU A 113 6.053 6.985 7.724 1.00 66.64 O
ATOM 375 CB LEU A 113 5.000 4.344 5.969 1.00 68.64 C
ATOM 376 CG LEU A 113 3.706 3.716 5.413 1.00 64.30 C
ATOM 377 CD1 LEU A 113 3.811 2.195 5.483 1.00 61.60 C
ATOM 378 CD2 LEU A 113 2.476 4.186 6.176 1.00 60.67 C
ATOM 379 N GLY A 114 7.799 6.894 6.390 1.00 79.56 N
ATOM 380 CA GLY A 114 9.086 7.227 6.998 1.00 79.03 C
ATOM 381 C GLY A 114 9.990 6.018 7.269 1.00 79.25 C
ATOM 382 O GLY A 114 10.863 6.092 8.137 1.00 76.11 O
ATOM 383 N ARG A 115 6.544 3.930 8.072 1.00 74.09 N
ATOM 384 CA ARG A 115 7.561 4.343 9.045 1.00 74.39 C
ATOM 385 C ARG A 115 7.602 5.863 9.215 1.00 73.52 C
ATOM 386 O ARG A 115 7.688 6.333 10.339 1.00 73.25 O
ATOM 387 CB ARG A 115 8.941 3.818 8.615 1.00 73.92 C
ATOM 388 CG ARG A 115 9.132 2.320 8.894 1.00 70.53 C
ATOM 389 CD ARG A 115 10.532 1.828 8.468 1.00 69.22 C
ATOM 390 NE ARG A 115 10.666 1.734 7.004 1.00 64.65 N
ATOM 391 CZ ARG A 115 11.769 1.567 6.290 1.00 58.18 C
ATOM 392 NH1 ARG A 115 12.963 1.564 6.797 1.00 55.57 N
ATOM 393 NH2 ARG A 115 11.709 1.389 5.002 1.00 54.62 N
ATOM 394 N PRO A 116 14.717 5.226 5.524 1.00 53.61 N
ATOM 395 CA PRO A 116 14.047 4.005 5.122 1.00 54.53 C
ATOM 396 C PRO A 116 15.046 2.839 5.107 1.00 54.73 C
ATOM 397 O PRO A 116 16.095 2.961 4.468 1.00 53.64 O
ATOM 398 CB PRO A 116 13.445 4.283 3.739 1.00 52.81 C
ATOM 399 CG PRO A 116 14.305 5.421 3.191 1.00 50.59 C
ATOM 400 CD PRO A 116 14.717 6.194 4.442 1.00 53.27 C
ATOM 401 N ALA A 117 14.049 1.930 3.012 1.00 63.16 N
ATOM 402 CA ALA A 117 13.633 1.344 1.737 1.00 62.67 C
ATOM 403 C ALA A 117 14.430 0.072 1.401 1.00 61.46 C
ATOM 404 O ALA A 117 15.655 0.045 1.537 1.00 57.80 O
ATOM 405 CB ALA A 117 13.785 2.403 0.635 1.00 59.41 C
ATOM 406 N ALA A 118 15.805 0.122 1.763 1.00 55.43 N
ATOM 407 CA ALA A 118 15.891 -0.403 0.410 1.00 56.39 C
ATOM 408 C ALA A 118 15.048 -1.680 0.305 1.00 56.04 C
ATOM 409 O ALA A 118 15.326 -2.672 0.984 1.00 53.91 O
ATOM 410 CB ALA A 118 17.362 -0.642 0.057 1.00 53.50 C
ATOM 411 N THR A 119 15.121 -1.353 -1.496 1.00 61.38 N
ATOM 412 CA THR A 119 16.127 -1.899 -2.411 1.00 60.53 C
ATOM 413 C THR A 119 15.522 -1.905 -3.807 1.00 59.67 C
ATOM 414 O THR A 119 14.421 -2.422 -3.979 1.00 55.06 O
ATOM 415 CB THR A 119 16.541 -3.316 -1.985 1.00 57.34 C
ATOM 416 CG2 THR A 119 17.381 -3.276 -0.701 1.00 49.04 C
ATOM 417 OG1 THR A 119 15.411 -4.115 -1.725 1.00 50.65 O
ATOM 418 N GLU A 120 16.604 -1.923 -4.145 1.00 50.99 N
ATOM 419 CA GLU A 120 17.204 -3.187 -4.574 1.00 54.34 C
ATOM 420 C GLU A 120 17.851 -3.021 -5.956 1.00 55.71 C
ATOM 421 O GLU A 120 17.201 -2.522 -6.877 1.00 54.52 O
ATOM 422 CB GLU A 120 16.195 -4.346 -4.565 1.00 52.07 C
ATOM 423 CG GLU A 120 15.869 -4.827 -3.141 1.00 46.57 C
ATOM 424 CD GLU A 120 15.156 -6.193 -3.123 1.00 43.72 C
ATOM 425 OE1 GLU A 120 15.265 -6.874 -2.075 1.00 42.01 O
ATOM 426 OE2 GLU A 120 14.510 -6.549 -4.127 1.00 40.56 O
ATOM 427 N SER A 121 15.484 -2.963 -7.368 1.00 54.21 N
ATOM 428 CA SER A 121 14.704 -3.561 -8.463 1.00 55.29 C
ATOM 429 C SER A 121 15.238 -4.927 -8.888 1.00 56.87 C
ATOM 430 O SER A 121 16.454 -5.133 -8.898 1.00 54.98 O
ATOM 431 CB SER A 121 14.745 -2.643 -9.688 1.00 51.48 C
ATOM 432 OG SER A 121 16.101 -2.511 -10.117 1.00 45.49 O
ATOM 433 N GLU A 122 15.850 -5.282 -9.605 1.00 51.77 N
ATOM 434 CA GLU A 122 16.182 -6.564 -10.249 1.00 55.13 C
ATOM 435 C GLU A 122 16.998 -6.373 -11.538 1.00 57.01 C
ATOM 436 O GLU A 122 16.839 -5.383 -12.259 1.00 55.47 O
ATOM 437 CB GLU A 122 14.859 -7.306 -10.493 1.00 52.76 C
ATOM 438 CG GLU A 122 15.016 -8.779 -10.889 1.00 46.74 C
ATOM 439 CD GLU A 122 13.662 -9.526 -10.923 1.00 44.68 C
ATOM 440 OE1 GLU A 122 13.693 -10.756 -11.076 1.00 42.29 O
ATOM 441 OE2 GLU A 122 12.601 -8.856 -10.788 1.00 41.78 O
ATOM 442 N ALA A 123 17.354 -7.449 -12.003 1.00 60.26 N
ATOM 443 CA ALA A 123 18.183 -7.449 -13.204 1.00 63.33 C
ATOM 444 C ALA A 123 17.358 -7.785 -14.455 1.00 64.78 C
ATOM 445 O ALA A 123 16.457 -8.617 -14.405 1.00 59.86 O
ATOM 446 CB ALA A 123 19.350 -8.433 -13.001 1.00 60.03 C
ATOM 447 N GLY A 124 18.523 -8.096 -13.719 1.00 63.07 N
ATOM 448 CA GLY A 124 17.859 -8.329 -15.011 1.00 65.23 C
ATOM 449 C GLY A 124 16.694 -7.387 -15.324 1.00 68.49 C
ATOM 450 O GLY A 124 16.186 -7.401 -16.446 1.00 64.05 O
ATOM 451 N CYS A 125 14.643 -4.953 -13.378 1.00 55.99 N
ATOM 452 CA CYS A 125 13.696 -4.111 -14.105 1.00 57.24 C
ATOM 453 C CYS A 125 13.998 -2.621 -13.896 1.00 58.70 C
ATOM 454 O CYS A 125 13.100 -1.823 -13.595 1.00 55.87 O
ATOM 455 CB CYS A 125 12.263 -4.512 -13.747 1.00 55.32 C
ATOM 456 SG CYS A 125 11.869 -6.177 -14.363 1.00 50.74 S
ATOM 457 N GLU A 126 14.772 -3.582 -14.020 1.00 59.68 N
ATOM 458 CA GLU A 126 15.265 -2.206 -13.824 1.00 61.58 C
ATOM 459 C GLU A 126 14.472 -1.166 -14.633 1.00 63.24 C
ATOM 460 O GLU A 126 14.255 -0.037 -14.176 1.00 61.69 O
ATOM 461 CB GLU A 126 16.762 -2.192 -14.167 1.00 59.28 C
ATOM 462 CG GLU A 126 17.436 -0.858 -13.783 1.00 53.24 C
ATOM 463 CD GLU A 126 18.956 -0.867 -13.972 1.00 49.84 C
ATOM 464 OE1 GLU A 126 19.580 0.113 -13.510 1.00 45.41 O
ATOM 465 OE2 GLU A 126 19.488 -1.816 -14.588 1.00 44.49 O
ATOM 466 N TRP A 127 14.298 0.197 -15.353 1.00 65.80 N
ATOM 467 CA TRP A 127 13.451 1.065 -16.166 1.00 66.51 C
ATOM 468 C TRP A 127 12.182 1.555 -15.433 1.00 66.23 C
ATOM 469 O TRP A 127 11.689 2.641 -15.736 1.00 65.50 O
ATOM 470 CB TRP A 127 13.076 0.332 -17.456 1.00 65.42 C
ATOM 471 CG TRP A 127 12.106 -0.794 -17.293 1.00 63.96 C
ATOM 472 CD1 TRP A 127 12.418 -2.083 -17.036 1.00 58.31 C
ATOM 473 CD2 TRP A 127 10.646 -0.736 -17.349 1.00 61.97 C
ATOM 474 CE2 TRP A 127 10.140 -2.044 -17.104 1.00 59.81 C
ATOM 475 CE3 TRP A 127 9.710 0.291 -17.577 1.00 57.19 C
ATOM 476 NE1 TRP A 127 11.260 -2.828 -16.923 1.00 54.93 N
ATOM 477 CZ2 TRP A 127 8.759 -2.317 -17.092 1.00 59.98 C
ATOM 478 CZ3 TRP A 127 8.323 0.025 -17.564 1.00 57.53 C
ATOM 479 CH2 TRP A 127 7.856 -1.275 -17.319 1.00 56.51 C
ATOM 480 N LEU A 128 12.164 0.017 -14.197 1.00 67.05 N
ATOM 481 CA LEU A 128 11.042 0.462 -13.350 1.00 67.30 C
ATOM 482 C LEU A 128 11.406 1.679 -12.493 1.00 66.35 C
ATOM 483 O LEU A 128 10.518 2.422 -12.081 1.00 63.62 O
ATOM 484 CB LEU A 128 10.608 -0.684 -12.422 1.00 67.27 C
ATOM 485 CG LEU A 128 9.894 -1.849 -13.111 1.00 65.13 C
ATOM 486 CD1 LEU A 128 9.750 -3.014 -12.127 1.00 61.56 C
ATOM 487 CD2 LEU A 128 8.492 -1.451 -13.562 1.00 62.62 C
ATOM 488 N GLN A 129 12.310 2.314 -12.678 1.00 60.28 N
ATOM 489 CA GLN A 129 12.750 3.593 -12.121 1.00 62.88 C
ATOM 490 C GLN A 129 12.595 4.743 -13.131 1.00 64.31 C
ATOM 491 O GLN A 129 12.333 5.878 -12.748 1.00 64.10 O
ATOM 492 CB GLN A 129 14.210 3.474 -11.649 1.00 61.08 C
ATOM 493 CG GLN A 129 14.385 2.411 -10.557 1.00 55.15 C
ATOM 494 CD GLN A 129 15.818 2.309 -10.028 1.00 49.34 C
ATOM 495 NE2 GLN A 129 16.104 1.347 -9.196 1.00 43.39 N
ATOM 496 OE1 GLN A 129 16.696 3.094 -10.332 1.00 47.13 O
ATOM 497 N GLY A 130 13.200 3.927 -14.102 1.00 77.71 N
ATOM 498 CA GLY A 130 13.153 5.143 -14.909 1.00 78.59 C
ATOM 499 C GLY A 130 11.921 5.292 -15.805 1.00 81.12 C
ATOM 500 O GLY A 130 11.786 6.343 -16.427 1.00 77.24 O
ATOM 501 N ALA A 131 10.898 5.170 -15.866 1.00 68.35 N
ATOM 502 CA ALA A 131 9.637 5.017 -16.567 1.00 68.77 C
ATOM 503 C ALA A 131 8.710 6.228 -16.342 1.00 68.93 C
ATOM 504 O ALA A 131 8.199 6.432 -15.233 1.00 67.51 O
ATOM 505 CB ALA A 131 8.969 3.707 -16.141 1.00 66.92 C
ATOM 506 N PRO A 132 8.919 6.281 -17.233 1.00 67.63 N
ATOM 507 CA PRO A 132 8.043 7.436 -17.113 1.00 68.72 C
ATOM 508 C PRO A 132 6.618 7.051 -16.705 1.00 69.83 C
ATOM 509 O PRO A 132 5.935 7.826 -16.049 1.00 67.02 O
ATOM 510 CB PRO A 132 8.095 8.124 -18.477 1.00 67.25 C
ATOM 511 CG PRO A 132 8.455 6.994 -19.445 1.00 64.94 C
ATOM 512 CD PRO A 132 9.362 6.105 -18.603 1.00 68.34 C
ATOM 513 N LEU A 133 6.261 6.874 -17.781 1.00 70.85 N
ATOM 514 CA LEU A 133 5.264 6.236 -16.914 1.00 73.64 C
ATOM 515 C LEU A 133 4.900 7.142 -15.743 1.00 74.98 C
ATOM 516 O LEU A 133 5.429 7.040 -14.636 1.00 73.93 O
ATOM 517 CB LEU A 133 5.739 4.829 -16.517 1.00 72.99 C
ATOM 518 CG LEU A 133 5.022 3.744 -17.344 1.00 69.53 C
ATOM 519 CD1 LEU A 133 6.028 2.775 -17.939 1.00 64.63 C
ATOM 520 CD2 LEU A 133 4.032 2.968 -16.491 1.00 65.81 C
ATOM 521 N LEU A 134 6.860 5.829 -15.091 1.00 70.85 N
ATOM 522 CA LEU A 134 5.863 5.191 -14.224 1.00 73.64 C
ATOM 523 C LEU A 134 5.499 6.097 -13.053 1.00 74.98 C
ATOM 524 O LEU A 134 6.028 5.995 -11.946 1.00 73.93 O
ATOM 525 CB LEU A 134 6.338 3.784 -13.827 1.00 72.99 C
ATOM 526 CG LEU A 134 5.621 2.699 -14.654 1.00 69.53 C
ATOM 527 CD1 LEU A 134 6.627 1.730 -15.249 1.00 64.63 C
ATOM 528 CD2 LEU A 134 4.631 1.923 -13.801 1.00 65.81 C
ATOM 529 N ARG A 135 5.563 7.166 -12.858 1.00 69.05 N
ATOM 530 CA ARG A 135 5.328 8.534 -12.352 1.00 69.64 C
ATOM 531 C ARG A 135 3.846 8.881 -12.292 1.00 70.46 C
ATOM 532 O ARG A 135 3.474 9.754 -11.526 1.00 69.07 O
ATOM 533 CB ARG A 135 6.072 9.559 -13.221 1.00 68.24 C
ATOM 534 CG ARG A 135 7.588 9.317 -13.253 1.00 62.97 C
ATOM 535 CD ARG A 135 8.267 10.379 -14.117 1.00 59.05 C
ATOM 536 NE ARG A 135 9.715 10.130 -14.216 1.00 54.76 N
ATOM 537 CZ ARG A 135 10.587 10.841 -14.913 1.00 46.01 C
ATOM 538 NH1 ARG A 135 10.222 11.892 -15.594 1.00 45.30 N
ATOM 539 NH2 ARG A 135 11.840 10.496 -14.940 1.00 44.74 N
ATOM 540 N ALA A 136 4.154 8.388 -14.079 1.00 86.83 N
ATOM 541 CA ALA A 136 2.720 8.561 -14.198 1.00 87.61 C
ATOM 542 C ALA A 136 1.910 7.791 -13.136 1.00 88.66 C
ATOM 543 O ALA A 136 0.702 7.997 -13.043 1.00 86.73 O
ATOM 544 CB ALA A 136 2.313 8.171 -15.631 1.00 86.55 C
ATOM 545 N LEU A 137 2.709 6.668 -11.467 1.00 78.13 N
ATOM 546 CA LEU A 137 1.935 5.871 -10.522 1.00 81.01 C
ATOM 547 C LEU A 137 0.944 6.744 -9.744 1.00 82.26 C
ATOM 548 O LEU A 137 1.285 7.869 -9.357 1.00 81.20 O
ATOM 549 CB LEU A 137 2.894 5.130 -9.576 1.00 80.19 C
ATOM 550 CG LEU A 137 3.661 3.984 -10.259 1.00 77.22 C
ATOM 551 CD1 LEU A 137 4.810 3.521 -9.364 1.00 73.59 C
ATOM 552 CD2 LEU A 137 2.762 2.776 -10.517 1.00 73.10 C
ATOM 553 N PRO A 138 -0.202 6.284 -9.576 1.00 84.33 N
ATOM 554 CA PRO A 138 -1.166 7.028 -8.793 1.00 84.92 C
ATOM 555 C PRO A 138 -0.632 7.283 -7.375 1.00 86.19 C
ATOM 556 O PRO A 138 0.039 6.422 -6.790 1.00 85.23 O
ATOM 557 CB PRO A 138 -2.454 6.181 -8.796 1.00 82.95 C
ATOM 558 CG PRO A 138 -1.933 4.763 -8.985 1.00 83.36 C
ATOM 559 CD PRO A 138 -0.702 4.948 -9.877 1.00 86.54 C
ATOM 560 N ASP A 139 -1.526 8.369 -6.677 1.00 70.86 N
ATOM 561 CA ASP A 139 -0.872 8.805 -5.450 1.00 71.19 C
ATOM 562 C ASP A 139 -1.139 7.841 -4.297 1.00 69.72 C
ATOM 563 O ASP A 139 -2.261 7.349 -4.088 1.00 67.52 O
ATOM 564 CB ASP A 139 -1.314 10.230 -5.062 1.00 69.01 C
ATOM 565 CG ASP A 139 -0.809 11.279 -6.050 1.00 63.08 C
ATOM 566 OD1 ASP A 139 0.388 11.204 -6.408 1.00 57.64 O
ATOM 567 OD2 ASP A 139 -1.627 12.147 -6.428 1.00 56.76 O
ATOM 568 N PHE A 140 -0.716 7.803 -3.530 1.00 83.67 N
ATOM 569 CA PHE A 140 -1.329 7.085 -2.422 1.00 84.93 C
ATOM 570 C PHE A 140 -2.379 7.960 -1.719 1.00 85.69 C
ATOM 571 O PHE A 140 -2.244 9.187 -1.658 1.00 83.80 O
ATOM 572 CB PHE A 140 -0.267 6.592 -1.438 1.00 83.59 C
ATOM 573 CG PHE A 140 0.582 5.449 -1.961 1.00 83.70 C
ATOM 574 CD1 PHE A 140 -0.009 4.183 -2.170 1.00 79.84 C
ATOM 575 CD2 PHE A 140 1.943 5.624 -2.233 1.00 80.30 C
ATOM 576 CE1 PHE A 140 0.767 3.115 -2.648 1.00 78.37 C
ATOM 577 CE2 PHE A 140 2.722 4.553 -2.708 1.00 78.77 C
ATOM 578 CZ PHE A 140 2.134 3.297 -2.917 1.00 80.56 C
ATOM 579 N PRO A 141 -3.377 7.146 -1.218 1.00 85.62 N
ATOM 580 CA PRO A 141 -4.293 7.851 -0.332 1.00 84.83 C
ATOM 581 C PRO A 141 -3.547 8.411 0.887 1.00 84.90 C
ATOM 582 O PRO A 141 -2.582 7.814 1.345 1.00 83.06 O
ATOM 583 CB PRO A 141 -5.349 6.818 0.076 1.00 82.95 C
ATOM 584 CG PRO A 141 -5.264 5.751 -1.009 1.00 82.97 C
ATOM 585 CD PRO A 141 -3.790 5.774 -1.405 1.00 86.27 C
ATOM 586 N ALA A 142 -3.693 10.544 1.433 1.00 79.68 N
ATOM 587 CA ALA A 142 -3.179 10.949 2.732 1.00 77.99 C
ATOM 588 C ALA A 142 -3.258 9.791 3.743 1.00 75.71 C
ATOM 589 O ALA A 142 -4.303 9.153 3.902 1.00 71.99 O
ATOM 590 CB ALA A 142 -3.942 12.185 3.218 1.00 75.46 C
ATOM 591 N ASP A 143 -5.888 8.670 2.765 1.00 78.61 N
ATOM 592 CA ASP A 143 -5.442 9.116 4.086 1.00 77.23 C
ATOM 593 C ASP A 143 -5.180 7.896 4.985 1.00 75.85 C
ATOM 594 O ASP A 143 -5.997 6.969 5.066 1.00 73.35 O
ATOM 595 CB ASP A 143 -6.466 10.072 4.727 1.00 74.80 C
ATOM 596 CG ASP A 143 -6.543 11.450 4.053 1.00 67.16 C
ATOM 597 OD1 ASP A 143 -5.500 11.940 3.572 1.00 61.12 O
ATOM 598 OD2 ASP A 143 -7.647 12.041 4.058 1.00 60.16 O
ATOM 599 N PHE A 144 -3.219 6.486 6.110 1.00 64.38 N
ATOM 600 CA PHE A 144 -3.807 6.622 7.432 1.00 62.92 C
ATOM 601 C PHE A 144 -2.728 6.413 8.504 1.00 63.08 C
ATOM 602 O PHE A 144 -1.722 5.734 8.293 1.00 59.57 O
ATOM 603 CB PHE A 144 -5.000 5.650 7.582 1.00 58.83 C
ATOM 604 CG PHE A 144 -4.624 4.185 7.510 1.00 55.99 C
ATOM 605 CD1 PHE A 144 -4.444 3.561 6.260 1.00 51.90 C
ATOM 606 CD2 PHE A 144 -4.418 3.460 8.691 1.00 53.55 C
ATOM 607 CE1 PHE A 144 -4.043 2.221 6.199 1.00 46.26 C
ATOM 608 CE2 PHE A 144 -4.013 2.116 8.630 1.00 49.76 C
ATOM 609 CZ PHE A 144 -3.820 1.496 7.380 1.00 49.04 C
ATOM 610 N THR A 145 -3.378 8.753 10.308 1.00 68.53 N
ATOM 611 CA THR A 145 -2.826 8.090 11.491 1.00 67.14 C
ATOM 612 C THR A 145 -1.707 7.175 11.023 1.00 66.09 C
ATOM 613 O THR A 145 -1.800 6.554 9.968 1.00 62.96 O
ATOM 614 CB THR A 145 -3.921 7.315 12.249 1.00 64.21 C
ATOM 615 CG2 THR A 145 -4.736 8.256 13.130 1.00 55.51 C
ATOM 616 OG1 THR A 145 -4.816 6.693 11.357 1.00 56.56 O
ATOM 617 N LEU A 146 -0.988 4.076 8.997 1.00 59.75 N
ATOM 618 CA LEU A 146 0.038 3.044 8.822 1.00 58.74 C
ATOM 619 C LEU A 146 -0.683 1.699 8.771 1.00 59.64 C
ATOM 620 O LEU A 146 -1.156 1.229 9.807 1.00 56.56 O
ATOM 621 CB LEU A 146 1.027 3.104 10.004 1.00 54.64 C
ATOM 622 CG LEU A 146 2.316 3.896 9.694 1.00 49.97 C
ATOM 623 CD1 LEU A 146 2.792 4.659 10.919 1.00 46.72 C
ATOM 624 CD2 LEU A 146 3.430 2.956 9.244 1.00 45.62 C
ATOM 625 N PRO A 147 -1.446 2.595 12.275 1.00 55.77 N
ATOM 626 CA PRO A 147 -0.846 1.613 13.127 1.00 55.53 C
ATOM 627 C PRO A 147 -0.436 0.478 12.196 1.00 55.69 C
ATOM 628 O PRO A 147 -1.284 -0.207 11.641 1.00 54.18 O
ATOM 629 CB PRO A 147 -1.912 1.230 14.159 1.00 53.11 C
ATOM 630 CG PRO A 147 -3.229 1.553 13.453 1.00 50.96 C
ATOM 631 CD PRO A 147 -2.880 2.722 12.536 1.00 52.42 C
ATOM 632 N PRO A 148 -0.455 1.437 17.955 1.00 52.39 N
ATOM 633 CA PRO A 148 0.795 1.011 18.545 1.00 53.35 C
ATOM 634 C PRO A 148 0.859 -0.523 18.561 1.00 53.80 C
ATOM 635 O PRO A 148 -0.091 -1.173 18.988 1.00 52.38 O
ATOM 636 CB PRO A 148 0.817 1.618 19.955 1.00 51.15 C
ATOM 637 CG PRO A 148 -0.663 1.844 20.274 1.00 49.36 C
ATOM 638 CD PRO A 148 -1.270 2.160 18.914 1.00 51.63 C
TER 639 PRO A 148
END
static/good-c-term-full.fasta 0 → 100644
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Name,fw/rv,fuction,length (bp),Anealing T,sequence,Secondary function,,,
iGEM01,fw,fw_Sc_ymNG_N_terminal_amplification,25,56,GTGTCTAAGGGTGAAGAAGATAACA,This is rv technically,,,
iGEM02,rv,rv_Sc_ymNG_N_terminal_amplification,25,54,CATAGATCTTATTTTATTGTATTGATTGTTGTTTT,This is fw technically,,,
iGEM03,rv,rv_Sc_ymNG_C_terminal_amplification,30,59,TAACTCGAGAGCTTTTGATTAAGCCTTCTA,,,,
,,rv_Sc_ymNG_C_terminal_amplification**,25,56,TAACTCGAGAGCTTTTGATTAAGCC,Could be better annealing,,,
iGEM04,fw,fw_Sc_ymNG_C_terminal_amplification,30,63,GGATCCCTTGTACAATTCGTCCATACCCAT,,,,
iGEM05,fw,fw_Sc_Nterminal_insert_amplification,25,53,TAGCAAAAACAACAATCAATACAAT,fw_dg_Nterminal insert,,,
iGEM06,rv,rv_Sc_Nterminal_insert_amplification,18,55,CTGGCAAAGAAGCCATGT,,,,
iGEM07,rv,rv_dg_Sc_Nterminal,20,58,GATACACGCGTCACCACAAG,,,,
iGEM08,fw,fw_dg_Sc_Cterminal,20,54,ATGGTGTCTAAGGGTGAAGA,,,,
iGEM09,fw,fw_Sc_Cterminal_insert_amplification,20,60,CACCGACGTCATGGGTATGG,,,,
iGEM10,rv,rv_Sc_Cterminal_insert_amplification,25,54,TTTTTTGGACTAGAAGGCTTAATCA,rv_dg_Sc_Cterminal,,,
iGEM11,fw,fw_Sc_Cterminal_PTS1_amp,39,63,tccaaattgGGATCCCTTGTACAATTCGTCCATACCCAT,,,,
iGEM12,rv,rv_Sc_Cterminal_PTS1_amp,39,59,caatttggaTAACTCGAGAGCTTTTGATTAAGCCTTCTA,,,,
iGEM13,fw,fw_Cr_ C terminal amplification,20,58,atcgagggcagggatatcga,,,,
iGEM14,rv,rv_Cr_ C terminal amplification,20,57,cttgtacagctcgtccatgc,,,,
iGEM15,fw,fw_Cr_ N terminal amplification,24,58,atgcatatgggatccagatctgac,,,,
iGEM16,rv,rv_Cr_ N terminal amplification,28,56,cattttaagatgttgagtgacttctctt,,,,
iGEM17,fw,fw_Cr_ C term amp PTS1,50,78,ATCCACCATCCCCGGGAGCTGAGCCGCCTGatcgagggcagggatatcga,,,,
iGEM18,rv,rv_Cr_ C term amp PTS1,50,75,CAGGCGGCTCAGCTCCCGGGGATGGTGGATcttgtacagctcgtccatgc,,,,
iGEM19,fw,fw_dg_Cr_Nterminal,20,67,gcggtggcggccgctctaga,,,,
iGEM20,rv,rv_dg_Cr_Nterminal,22,59,cgtcgtccttgaagaagatggt,,,,
iGEM21,fw,fw_dg_Cr_Cterminal,20,64,gtgagcaagggcgaggagct,,,,
iGEM22,fw,fw_Cr_Cterminal_insert_amplification,18,68,cgccgccggcatcaccct,,,,
iGEM23,rv,rv_Cr_Cterminal_insert_amplification,20,64,actgcgggtggctccagaat,rv_dg_Cr_Cterminal,,,
iGEM24,fw,fw_Cr_Nterminal_insert_amplification,29,56,ttacttttttacaagagaagtcactcaac,,,,
iGEM25,rv,rv_Cr_Nterminal_insert_amplification,16,55,ccctgccctcgatgac,,,,
5-7-2024,,,,,,,,,
iGEM26,fw,fw Cr dg insert mVenus_ble,19,62,gtgagcaagggcgaggagc,,,,
iGEM27,rv,rv Cr dg insert mVenus_ble,21,62,ttagtcctgctcctcggccac,,,,
iGEM28,rv,rv_Sc_Cterminal_PTS1_phosphorilated,39,59,caatttggaTAACTCGAGAGCTTTTGATTAAGCCTTCTA,"The same as the one above, just phosphorilated",,,
iGEM29,rv,rv_Cr_ C term amp PTS1_phosphorilated,25,61,CAGGCGGCTCAGCTCCCGGGGATGGTGGATcttgtacagctcgtccatgc,Here the G should not matter,,,
iGEM30,fw,fw_Cr_ C terminal amplification_phos,20,58,atcgagggcagggatatcga,"The same as the one above, just phosphorilated",,,
iGEM31,fw,fw_Sc_ymNG_C_terminal_amplification,30,59,TAACTCGAGAGCTTTTGATTAAGCCTTCTA,"The same as the one above, just phosphorilated",,,wrong order!!
iGEM32,fw,fw_Sc_ymNG_C_terminal_amplification,28,62,GGATCCCTTGTACAATTCGTCCATACCC,should be this,,,
\ No newline at end of file
wiki/pages/experiments.html
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......@@ -16,10 +16,11 @@
<div onclick="goTo(document.getElementById('three'))"><span id="subtitle3" style="color: #62D881;">Plasmid Design</span></div>
<div onclick="goTo(document.getElementById('four'))"><span id="subtitle4" style="color: #62D881;"><i>E. coli</i></span></div>
<div onclick="goTo(document.getElementById('five'))"><span id="subtitle5" style="color: #62D881;"><i>S.cerevisiae </i></span></div>
<div onclick="goTo(document.getElementById('six'))"><span id="subtitle5" style="color: #62D881;"><i>C. reinhardtii</i></span></div>
<div onclick="goTo(document.getElementById('seven'))"><span id="subtitle5" style="color: #62D881;">PEG Fusion</span></div>
<div onclick="goTo(document.getElementById('eight'))"><span id="subtitle5" style="color: #62D881;">Protocols</span></div>
<div onclick="goTo(document.getElementById('nine'))"><span id="subtitle5" style="color: #62D881;">References</span></div>
<div onclick="goTo(document.getElementById('six'))"><span id="subtitle6" style="color: #62D881;"><i>C. reinhardtii</i></span></div>
<div onclick="goTo(document.getElementById('seven'))"><span id="subtitle7" style="color: #62D881;">PEG Fusion</span></div>
<div onclick="goTo(document.getElementById('eight'))"><span id="subtitle8" style="color: #62D881;">Data and Code</span></div>
<div onclick="goTo(document.getElementById('nine'))"><span id="subtitle9" style="color: #62D881;">Protocols</span></div>
<div onclick="goTo(document.getElementById('ten'))"><span id="subtitle10" style="color: #62D881;">References</span></div>
</div>
<div class="pagecontent">
......@@ -39,7 +40,7 @@
<!-- 2 -->
<div class="h" id="two">
<div class="h1"><i>B. bigelowii</i> Proteome Analysis</div>
<p>Using raw RNA-seq data from <a href="#cite27">[27]</a>, a transcriptome assembly was constructed using DRAP <a href="#cite35">[35]</a> with the oases assembler and default parameters. The completeness of the assembly was tested with BUSCO <a href="#cite33">[33]</a>. Proteins were predicted from the transcriptome assembly with TransDecoder <a href="#cite34">[34]</a>. Ortholog analysis was performed using OrthoFinder <a href="#cite36">[36]</a>.</p>
<p>Using raw RNA-seq data from <a href="#cite27">[27]</a>, a transcriptome assembly was constructed using DRAP <a href="#cite35">[35]</a> with the oases assembler and default parameters. The completeness of the assembly was tested with BUSCO <a href="#cite33">[33]</a>. Proteins were predicted from the transcriptome assembly with TransDecoder <a href="#cite34">[34]</a>. Ortholog analysis was performed using OrthoFinder <a href="#cite36">[36]</a>. See <a href="#eight">Data and Code</a> for the predicted proteome.</p>
</div>
<!-- 3 -->
......@@ -49,6 +50,7 @@
<p>Two existing vectors with fluorescent proteins were used as backbones for this study. For <i>S. cerevisiae</i>, we utilized pUDE1311 <a href="#cite11">[11]</a>, which contains mNeonGreen and a URA3 marker for selection (full genotype: ConLS-ScHHF2p-ymNeonGreen-ScENO1t-ConR1-URA3-2µ-AmpR). This vector was kindly provided by Marcel Vieira Lara of TU Delft. For <i>C. reinhardtii</i>, we used pOpt2 <a href="#cite9">[9]</a> <a href="#cite10">[10]</a>, containing mVenus and a ble marker for selection. This plasmid, kindly provided by Friedrich Kleiner from the MEA lab at TU Delft, also features specific introns in the mVenus coding sequence to prevent gene silencing. Both plasmids contain an ampicillin resistance gene for selection in <i>E. coli</i>. The complete sequences of both unmodified vectors and plasmids with all inserts have been made publicly available [link].</p>
<p>All plasmids, inserts, and primers were designed in SnapGene (www.snapgene.com). The inserts containing the transit peptides were synthesized by GenScript (Piscataway, NJ, USA). Gibson Assembly was employed for plasmid construction. Primers with specific overhangs were designed for PCR amplification of inserts and linearization of plasmid backbones at both the C-terminal and N-terminal regions, for inserting uTP1/2 and MTS1/mTP/cTP respectively.</p>
<p>PCR amplification was performed using KOD polymerase. To eliminate leftover circular plasmid from the backbone amplification process, DpnI digestion was utilized. Gibson Assembly was then carried out to combine the linearized plasmid backbones with the uTP, MTS1, mTP and cTP inserts using the Gibson Assembly® Cloning Kit by New England Biolabs <a href="#cite1">[1]</a>, following the manufacturer’s protocol <a href="#cite2">[2]</a>. pUC19 and a DNA fragment provided by the kit were used as positive controls.</p>
<p>See <a href="/tu-delft/parts/">Parts and Sequences</a> for more details on the design and sequence of our parts.</p>
</div>
......@@ -88,8 +90,19 @@
<p>All samples were imaged using fluorescent scanning confocal microscopy (Nikon confocal A1R, excitation 488-nm@16mW, emission filter 525/50) to evaluate fusion events. Cells were segmented using the transmitted light channel and instances of bright fluorescent spots within cells were evaluated. Image analysis was performed in ImageJ.</p>
</div>
<div class="h" id="eight">
<div class="h1">Data and Code</div>
<p>We are making our code and data publicly available to help future iGEM teams who wish to build on our work.</p>
<p>The code used to characterize the uTP sequences is available on <a href="https://gitlab.igem.org/mablin/utp-sequence-analysis">this repository</a>.</p>
<p>The list of selected <em>B. bigelowii</em> protein sequences with C-terminal uTP can be downloaded <a href="{{ url_for('static', filename='good-c-term-fill.fasta') }}">here</a></p>
<p>The consensus structure obrained for uTP can be downloaded <a href="{{ url_for('static', filename='consensus-c-term.pdb') }}">here</a></p>
<p>Our predicted <em>B. bigelowii</em> proteome is available upon request.</p>
<p>The sequences of all primers used in our experiments can be found here <a href="{{ url_for('static', filename='primers.csv') }}">here</a></p>
<p>See <a href="/tu-delft/parts/">Parts and Sequences</a> for more details on the design and sequence of our parts.</p>
</div>
<!-- 8 -->
<div class="h" id="eight">
<div class="h" id="nine">
<div class="h1">Protocols</div>
<div class="h2">Yeast Transformation</div>
<p>Adapted from <a href="#cite7">[7]</a> following <a href="#cite8">[8]</a>.</p>
......@@ -383,7 +396,7 @@
<!-- 9 -->
<div class="h" id="nine">
<div class="h" id="ten">
<div class="h1">References</div>
<ol style="text-align:justify; font-family:AccidenzCommons; color:#185A4F; font-weight:400; font-size: min(1.5vw, 22px); font-style: normal; line-height: normal;">
<li id="cite1">New England Biolabs. (2024). Gibson Assembly® Cloning Kit | NEB. <a href="https://www.neb.com/en/products/e5510-gibson-assembly-cloning-kit" style="color:#185A4F;">https://www.neb.com/en/products/e5510-gibson-assembly-cloning-kit</a></li>
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<p>To allow the close study of UCYN-A by future iGEM teams and lay some groundwork for UCYN-A transplantation efforts, we developed a new, easier protocol for isolating it from a culture of B. bigelowii, compared to the known method reported by <a href="#cite1" style="color: #185A4F;">[1]</a>. While Coale et al relied on a multistep procedure with a Percoll gradient and centrifugation, we used a sorting flow cytometer after lysing of the host cells. We confirmed UCYN-A’s presence in the isolate with PCR, with primers targeting part of the 16S rRNA gene exclusive to prokaryotes.
</p>
<div class="img-pagestyle">
<img src="https://static.igem.wiki/teams/5054/cytometry.png" alt="Fig 1: Graphical overview of the experiment plan.">
<img src="https://static.igem.wiki/teams/5054/cytometry-2.png" alt="Fig 1: Graphical overview of the experiment plan.">
<figcaption>Figure 12: Flow cytometry plot and PCR result for the three populations we identified on <em>B. bigelowii</em> lysate.
(<strong>a</strong>) shows the unlysed sample of <em>B. bigelowii</em>, (<strong>b</strong>) shows the lysed sample with the 3 populations marked for sorting, and (<strong>c</strong>) shows the PCR result for the 3 populations. The third population was hypothesized to contain the highest concentration of UCYN-A.
</figcaption>
</div>
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